
Cytology, Sputum









The Cytology, Sputum test involves a microscope examination of sputum (thick mucus coughed up from the lungs) to check for abnormal or cancerous cells. This test is commonly used to diagnose lung infections and respiratory diseases like chronic bronchitis. It also detects lung cancer.













Understanding Cytology, Sputum
Sputum is the thickened secretion produced in the lungs and the airways that is commonly coughed up during respiratory infections or illnesses. It contains mucus, dead cells, bacteria, and other materials of the respiratory tract. Sputum can provide important clues for the detection of diseases such as cancer, infection, and inflammation based on its composition and color. The Cytology, Sputum is a safe and non-invasive diagnostic test that detects abnormal cells in the respiratory tract, especially in the lungs. It studies the characteristic features of the sputum cells, such as size, shape, number, and arrangement, to determine abnormalities.
The Cytology, Sputum test is recommended for individuals presenting with symptoms such as persistent cough, pain in the chest, blood in the sputum (hemoptysis), weight loss without any known reason, or difficulty in breathing. Normally, it may be carried out in cases where the doctor suspects lung cancer, tuberculosis, or other respiratory diseases. In addition, the test is suggested for individuals with a high risk of lung cancer, such as smokers, people with a work history of exposure to harmful materials, like asbestos, or people with a family history of respiratory cancers. It may be used as an adjunct with other diagnostic tools, such as chest X-rays, chest CT scans, or bronchoscopies, to show the presence of abnormal cells or tumors in the lungs.
For this test, the individual needs to provide their clinical history, doctor's prescription, surgical and treatment history, and any available radiology findings. Make sure to fill out the Cytopathology Requisition Form, provided by the laboratory personnel. For sputum collection, a fresh, early morning sputum sample should be collected by coughing deeply for accurate analysis. To minimize contamination, rinse the mouth with water before sample collection.
Lab test results may vary across laboratories. Do not self-medicate at home solely based on these test results. Always consult the doctor for a proper understanding of the test results. The insights from the Cytology, Sputum test will help doctors prescribe targeted treatments, ensuring effective and appropriate medical care.
What does Cytology, Sputum measure?
The Cytology, Sputum test measures the presence of abnormal or malignant cells in sputum samples. It is tailored to identify potential lung cancers, infections, or other respiratory diseases by detecting the presence of atypical or cancerous cells. If cancerous cells are found, further diagnostic tests (such as imaging or biopsies) may be needed to determine the extent of the disease and to plan treatment. In addition to cancer, the detection of abnormal cells in sputum can also suggest lung infections or other respiratory conditions, such as tuberculosis (TB) or chronic obstructive pulmonary disease (COPD).
On the contrary, a negative test result means that no abnormal or malignant cells are found in the sputum sample. This suggests that the sample does not show signs of lung cancer, infections, or other significant lung diseases. However, a negative result does not completely rule out a condition, especially if symptoms persist. Sometimes, early-stage cancers or infections may not shed enough abnormal cells into the sputum, and additional tests or follow-ups may be needed to confirm or rule out a diagnosis.





FAQs related to Cytology, Sputum
- Shen F, Sergi C. Sputum Analysis. [Updated 2023 Feb 20]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2024 Jan-. Available from:
- Farzan S. Cough and Sputum Production. In: Walker HK, Hall WD, Hurst JW, editors. Clinical Methods: The History, Physical, and Laboratory Examinations. 3rd edition. Boston: Butterworths; 1990. Chapter 38. Available from:
- Petty TL. Sputum cytology for the detection of early lung cancer. Curr Opin Pulm Med. 2003 Jul;9(4):309-12. [Accessed 10 Dec. 2024].
- Lung Cancer—Patient Version [Internet]. NIH; National Cancer Institute. [Accessed 10 Dec. 2024]. Available from:
- Siddiqui F, Vaqar S, Siddiqui AH. Lung Cancer. [Updated 2023 May 8]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2024 Jan-. Available from:
- Ammanagi AS, Dombale VD, Miskin AT, Dandagi GL, Sangolli SS. Sputum cytology in suspected cases of carcinoma of lung (Sputum cytology a poor man's bronchoscopy!). Lung India. 2012 Jan;29(1):19-23. [Accessed 10 Dec. 2024]. Available from:
- Thunnissen FB. Sputum examination for early detection of lung cancer. J Clin Pathol. 2003 Nov;56(11):805-10. doi: 10.1136/jcp.56.11.805. PMID: 14600122; PMCID: PMC1770101. [Accessed 10 Dec. 2024]. Available from:
Other tests
- Allergy Panel (107 Allergens), LIA
- X - Ray Chest PA View
- Quantiferon®-TB Gold Plus (Interferon Gamma Release Assay)
- Sputum Culture and Sensitivity (Aerobic)
- AFB Staining, Sputum
- Viral Flu Panel (Influenza A&B, H3N2, H1N1, RSV) Qualitative RT-PCR
- Pulmonary Function Tests at Center (PFT)
- Gram Staining, Sputum
- TB Detection by GeneXpert Ultra MTB with Rifampicin Resistance, Sputum
- Coronavirus Covid -19 test- RT PCR
- CBC (Complete Blood Count)
- FBS (Fasting Blood Sugar)
- Thyroid Profile Total (T3, T4 & TSH)
- HbA1c (Glycosylated Hemoglobin) Test
- PPBS (Postprandial Blood Sugar)
- Lipid Profile
- Vitamin D (25-Hydroxy)
- Urine R/M (Urine Routine & Microscopy)
- Coronavirus Covid -19 test- RT PCR
- LFT (Liver Function Test)
- KFT (Kidney Function Test)
- TSH (Thyroid Stimulating Hormone) Ultrasensitive
- ESR (Erythrocyte Sedimentation Rate)
- Uric Acid, Serum
- Vitamin B12
- CRP (C-Reactive Protein), Quantitative
- Urine C/S (Urine Culture and Sensitivity)
- Serum Electrolytes
- Serum Calcium test
- Serum Creatinine
- Diabetes Screening (HbA1C & Fasting Sugar)
- KFT with Electrolytes (Kidney Function Test with Electrolytes)
- Cholesterol - Total
- Hb (Hemoglobin)
- Complete Hemogram (CBC & ESR)



















