
Vitamin D, B12 & Calcium










The Vitamin D, B12 & Calcium test measures the levels of vitamin D (25-Hydroxy), vitamin B12, and calcium in the blood. These tests are essential for evaluating bone strength, nerve function, and overall health. These tests help detect deficiencies or excesses that could lead to health problems.













Understanding Vitamin D, B12 & Calcium
The Vitamin D, B12 & Calcium test provides vital information about the overall health. Vitamin D (25-Hydroxy) is important for calcium absorption and bone strength while also supporting immune function and showing an association with mood regulation. Vitamin B12 plays an essential role in red blood cell formation, nerve function, and energy production. Calcium is a key mineral for strong bones and teeth, as well as for proper muscle and heart function. Together, these tests help identify deficiencies that could lead to conditions like osteoporosis, fatigue, nerve damage, or muscle weakness, ensuring timely intervention and better health outcomes.
Deficiencies of these nutrients can lead to symptoms like fatigue, muscle weakness, bone pain, numbness, or poor immune function. On the other hand, excessive levels can also cause problems such as kidney stones, nerve damage (excess vitamin B12), etc. The Vitamin D, B12 & Calcium profile helps detect imbalances early, ensuring proper management and preventing complications. It is especially important for individuals with bone health concerns, dietary restrictions, or frequent symptoms like fatigue, muscle weakness, etc.
Overnight fasting is preferred for the Vitamin D, B12 & Calcium test but is not mandatory. Inform your doctor about all the medications or supplements that you may be taking, as these may affect the test results, potentially requiring dosage adjustments or temporary discontinuation before the test.
Lab test results may vary across laboratories. Do not self-medicate at home solely based on these results, and always consult a doctor for a proper understanding of the test results. The test results will help them assess your health status, make recommendations for lifestyle modifications such as diet and exercise, prescribe medication if required to manage your condition, and formulate your overall treatment plan.
What does Vitamin D, B12 & Calcium measure?
Contains 3 testsThe Vitamin D, B12 & Calcium test measures the levels of vitamin D (25-Hydroxy), vitamin B12, and calcium in the blood. These are the important nutrients that are essential for overall health. Vitamin D (25-Hydroxy) is crucial for bone health and immune function. Vitamin B12 is necessary for energy production, nerve health, and red blood cell formation. Calcium is vital for strong bones, muscle function, and heart health. This profile helps detect imbalances, guiding appropriate treatments or the need for supplementation.
The Serum Calcium test measures the calcium level in your blood. Most of your body’s calcium (about 99%) is stored in bones and teeth to keep them strong. The rest (1%) circulates in the blood and helps your muscles move, nerves work, blood to clot, and heart to keep a steady rhythm.
Calcium in blood comes in two forms:
- Bound calcium, attached to proteins like albumin
- Ionized (free) calcium, which is active and helps many body functions
This test measures total calcium (both bound and ionized). This test cannot show if you have enough calcium in your diet. It also cannot detect bone diseases like osteoporosis. The body keeps blood calcium levels steady. It does this by taking calcium from bones during mild shortages.
Know more about Serum Calcium
The Vitamin B12 test measures the amount of vitamin B12 in your blood. Vitamin B12 is important for a healthy nervous system, making red blood cells, and helping in DNA formation. Low B12 levels are common in older adults, children, vegans or vegetarians, people with diabetes, those who had gastric bypass surgery, breastfeeding women, and people with conditions like Crohn’s disease that affect vitamin absorption. High B12 levels can occur in liver diseases, certain blood disorders, or from taking vitamin supplements. Usually, high levels from supplements are not harmful, as extra B12 is removed through urine.
Know more about Vitamin B12
The Vitamin D (25-Hydroxy) test measures the levels of vitamin D in the body. Vitamin D is an essential nutrient that can be synthesized in the body upon healthy exposure to sunlight or absorbed from dietary sources. It exists in two forms: vitamin D2 (ergocalciferol) and vitamin D3 (cholecalciferol). Vitamin D2 is present in plants, such as yeast or mushrooms, and is available as a supplement in fortified foods. Vitamin D3 is found in foods like cheese, mushrooms, egg yolks, and fatty fish.
Both forms of vitamin D need to be converted in the liver and kidneys before the body can use them effectively. This test shows the total level of vitamin D (D2 + D3) circulating in the body and guides your doctor in recommending the right treatment or lifestyle changes if needed.
Know more about Vitamin D (25-Hydroxy)





FAQs related to Vitamin D, B12 & Calcium
- Vitamin D [Internet]. NIH; 12 Aug. 2022 [Accessed 11 Sep. 2024]. Available from:
- Aparna P, Muthathal S, Nongkynrih B, Gupta SK. Vitamin D deficiency in India. J Family Med Prim Care. 2018 Mar-Apr;7(2):324-330. [Accessed 11 Sep. 2024]. Available from:
- Vitamin D [Internet]. Boston, USA: Harvard T.H. Chan; March 2023 [Accessed 11 Sep. 2024]. Available from:
- Vitamin B12 [Prescribing Information]. FDA; Jan. 2021 [Accessed 11 Sep. 2024]. Available from:
- Vitamin B12 or folate deficiency anemia. NHS; 20 Feb. 2023 [Accessed 11 Sep. 2024]. Available from:
- Duggan C, Srinivasan K, Thomas T, Samuel T, Rajendran R, Muthayya S, Finkelstein JL, Lukose A, Fawzi W, Allen LH, Bosch RJ, Kurpad AV. Vitamin B-12 supplementation during pregnancy and early lactation increases maternal, breast milk, and infant measures of vitamin B-12 status. J Nutr. 2014 May. [Accessed 11 Sep. 2024]. Available from:
- Gemici AI, Sevindik ÖG, Akar S, Tunca M. Vitamin B12 levels in familial Mediterranean fever patients treated with colchicine. Clin Exp Rheumatol. 2013. [Accessed 11 Sep. 2024]. Available from:
- Calcium [Internet]. NIH Office of Dietary Supplements; 06 Oct. 2022 [Accessed 11 Sep. 2024]. Available from:
- Osteoporosis [Internet]. NIA; 15 Nov. 2022 [Accessed 11 Sep. 2024]. Available from:
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- CRP (C-Reactive Protein), Quantitative
- Vitamin B12
- Magnesium
- TW-EYGDS HC
- Good Health Platinum Package with Smart Report
- Senior Citizen Health Checkup with Smart Report
- Men Health Checkup Premium with Smart Report
- Men Health Checkup Advanced with Smart Report
- Super Employee Health Check-up
- CBC (Complete Blood Count)
- FBS (Fasting Blood Sugar)
- Thyroid Profile Total (T3, T4 & TSH)
- HbA1c (Glycosylated Hemoglobin)
- PPBS (Postprandial Blood Sugar)
- Lipid Profile
- Vitamin D (25-Hydroxy)
- Urine R/M (Urine Routine & Microscopy)
- Coronavirus Covid -19 test- RT PCR
- LFT (Liver Function Test)
- KFT (Kidney Function Test)
- TSH (Thyroid Stimulating Hormone) Ultrasensitive
- ESR (Erythrocyte Sedimentation Rate)
- Uric Acid, Serum
- Vitamin B12
- CRP (C-Reactive Protein), Quantitative
- Urine C/S (Urine Culture and Sensitivity)
- Serum Electrolytes
- Serum Calcium
- Serum Creatinine
- Diabetes Screening (HbA1C & Fasting Sugar)
- KFT with Electrolytes (Kidney Function Test with Electrolytes)
- Cholesterol - Total
- Hb (Hemoglobin)
- Complete Hemogram (CBC & ESR)
























