
Vitamin D-1, 25-Dihydroxy









The Vitamin D-1, 25-Dihydroxy test is a blood test that helps measure the level of calcitriol, an active form of vitamin D responsible for maintaining proper calcium and phosphorus levels in the body. This test helps diagnose conditions related to vitamin D metabolism or bone disorders, such as osteoporosis or rickets. It is also used to monitor the effectiveness of vitamin D therapy.













Understanding Vitamin D-1, 25-Dihydroxy
Vitamin D helps maintain healthy bones by helping the body absorb calcium. It also plays a role in immune system function, nervous system maintenance, cell growth, and the reduction of inflammation. Additionally, it helps in maintaining stable levels of calcium and phosphorus in the blood.
1,25-Di Hydroxy Cholecalciferol (calcitriol) is the active form of vitamin D, produced in the kidneys. It plays a vital role in regulating calcium and phosphate levels in the body, which are important for healthy bones, teeth, and muscle function.
The Vitamin D-1, 25-Dihydroxy test is most commonly used to monitor conditions affecting vitamin D metabolism, like chronic kidney disease (CKD), hyperparathyroidism, and rickets.
A doctor may suggest the Vitamin D-1, 25-Dihydroxy test when there is a suspicion of abnormal calcium metabolism. It is particularly useful when other vitamin D tests (such as 25-hydroxy vitamin D) results are inconclusive. In cases of kidney disease, bone disorders, or a history of vitamin D deficiency, doctors might order this test to evaluate calcium balance and overall bone health. The test also helps doctors in assessing hyperparathyroidism, a condition where the parathyroid glands produce too much hormone, leading to abnormal calcium levels.
No special preparation is required for the Vitamin D-1, 25-Dihydroxy test. Inform the doctor about all the medications and supplements currently being taken, as some of them may affect the test results, potentially requiring dosage adjustments or temporary discontinuation before the test.
Test result ranges are approximate and may differ slightly between labs depending on the methodology and laboratory guidelines. Do not self-medicate, and always consult a doctor to understand the test results correctly. The results of the Vitamin D-1, 25-Dihydroxy test help doctors better understand how well the body is using it.
Please note: This is an outsourced test. The sample for this test is collected by TATA 1MG and processed at a NABL accredited partnered lab.
What does Vitamin D-1, 25-Dihydroxy measure?
The Vitamin D-1, 25-Dihydroxy test measures the level of calcitriol, the active form of vitamin D. This hormone plays essential roles in maintaining healthy bones and other body functions.
Low levels of calcitriol may occur in chronic renal impairment and also in cases of hypoparathyroidism, while high levels may occur in hyperparathyroidism or due to certain diseases like sarcoidosis and lymphomas.
The Vitamin D-1, 25-Dihydroxy test, along with other tests and medical history, helps your doctor determine if you have a vitamin D deficiency and advise on necessary treatment.





FAQs related to Vitamin D-1, 25-Dihydroxy
- Role of 1,25-Dihydroxycholecalciferol in Calcification of Bone and Maintenance of Serum Calcium Concentration in the Rat [Internet].ScienceDirect; [Accessed 04 Aug 2025]. Available From:
- Kumar R. Metabolism of 1,25-dihydroxyvitamin D3. Physiol Rev. 1984 Apr;64(2):478-504. [Accessed 04 Aug 2025]. Available From:
- PubChem. 1,25-Dihydroxyvitamin D3. PubChem Compound Database; CID 6437079. [Accessed 04 Aug 2025]. Available From:
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