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The Hepatitis B Surface Antigen (HBsAg) test detects HBsAg, a protein on the surface of the hepatitis B virus (HBV), in the blood. It is an important screening test for HBV infection. A reactive result needs follow-up with additional tests, such as anti-HBs and total anti-HBc antibodies, and can be confirmed with HBV DNA testing by PCR. This test helps identify individuals who may need medical treatment and supports measures to prevent the spread of hepatitis B. The test uses automated chemiluminescence immunoassay (CMIA/CLIA) technology, which may vary slightly across laboratories.

What Do the Results Mean?

Pathological Factors

Physiological Factors
 🧒 Age: Newborns and infants may have maternal HBsAg in their blood, which can affect test results.

🫄 Pregnancy: Changes in immunity and liver function during pregnancy may slightly affect HBsAg detection.

⏳ Infection Stage: Very early infection (window period) or late/chronic stages may change HBsAg levels.

Lifestyle Factors

🥗 Alcohol: Heavy drinking can affect liver function, which may indirectly influence test results.

💊 Medications: Antivirals or immunosuppressive drugs can lower viral levels, potentially altering HBsAg readings.

💉 Recent Vaccination: Hepatitis B vaccination may affect antibody levels but usually does not impact HBsAg detection.

Disclaimer: This information is for educational purposes only and should not replace professional medical advice, diagnosis, or treatment. Always consult your healthcare provider for personalized guidance.

What Do Negative Hepatitis B Surface Antigen (HBsAg) Results Mean?

A negative result usually means you do not have an active hepatitis B infection at the time of the test. It does not rule out a past infection or very early-stage infection.

What Do Positive Hepatitis B Surface Antigen (HBsAg) Results Mean?

A positive result means the hepatitis B virus is present in your blood. Early detection enables timely treatment, monitoring, and measures to prevent the spread to others.

What to Do if Your Hepatitis B Surface Antigen (HBsAg) Result Is Positive?

• Consult your doctor for further evaluation and treatment
• Inform close contacts so they can get vaccinated or tested
• Follow your doctor’s schedule for liver check-ups and viral load monitoring
• Avoid sharing personal items like razors or toothbrushes

Can Hepatitis B Surface Antigen (HBsAg) Results Be Inaccurate?

Though the test is reliable, certain factors may lead to false-positive or false-negative results:

• Low viral load (in the early stage
• Improper sample collection or handling
• Interference from recent vaccination or medical conditions

The Serum Creatinine test measures the creatinine level, a byproduct produced by the wear and tear of muscles during energy production. Since kidneys help filter creatinine, this test helps determine how well your kidneys work. Usually, high levels of creatinine in the blood signal diminished kidney function. At the same time, low levels may indicate decreased muscle mass.

The Microalbumin Creatinine Ratio, Urine test compares albumin and creatinine excretion in your urine. Typically, the body filters out creatinine in the urine steadily. Comparing the ratio of urine albumin with creatinine in the same urine sample helps analyze if the body is excreting albumin at an increased rate. Elevated levels indicate increased urinary excretion of albumin, which can be an early sign of kidney damage that may need medical intervention.

The Serum Albumin test measures the level of albumin protein in your blood. Albumin is one of the major proteins found in your body; about 60% of the total protein is made up of albumin, which is produced by the liver. It helps carry small molecules, such as hormones, vitamins, minerals, and medicines, throughout the body. It also serves as a source of amino acids for tissue metabolism. Albumin helps stop the fluid from leaking out of the blood vessels. When the albumin level is insufficient, the fluid can leak out of your blood vessels and build up in your lungs, belly, or other body parts.

The FBS (Fasting Blood Sugar) test measures the glucose level in the body under overnight fasting conditions. Glucose is the body's energy currency and is broken down through metabolism to produce energy. Hormones and enzymes produced by the liver and pancreas control this process. The hormone insulin, produced by the pancreas, regulates blood glucose levels. When these levels are high, such as after a meal, insulin is secreted to transport glucose into cells for energy production. Elevated glucose levels in the body after fasting may indicate a risk of developing prediabetes or diabetes, which can be of two types: Type 1, caused by little or no insulin production, and Type 2, caused by insulin resistance or decreased insulin production.

The Triglycerides test measures the amount of triglycerides in the blood and helps evaluate your risk of developing cardiovascular diseases. Triglycerides are a type of fat (lipid) that your body uses as a source of energy. When you consume more calories than your body needs, the excess calories are converted into triglycerides and stored in fat cells for later use. High triglyceride levels can contribute to the hardening and narrowing of arteries, increasing the risk of heart attack, stroke, and other related conditions. 

The Cholesterol - HDL test measures the concentration of high-density lipoprotein (HDL) cholesterol in the blood. HDL cholesterol plays a crucial role in maintaining cardiovascular health, as it helps transport excess low-density lipoprotein (LDL) cholesterol from the bloodstream back to the liver for excretion. This process prevents plaque buildup on the blood vessel walls, which can cause them to become narrow and less flexible. Higher HDL cholesterol levels are generally associated with a lower risk of heart problems, such as heart attacks and strokes. By measuring HDL cholesterol levels, your doctor can assess your risk of developing cardiovascular diseases and recommend appropriate preventive or treatment strategies, including lifestyle modifications and medications.

The Cholesterol - Total test measures the total amount of cholesterol (fats) in your blood. Cholesterol is mainly synthesized in the liver and partially in the intestines. It acts as a building block for cell membranes, is a precursor to vital hormones, and helps produce bile acids that help digest fats. Cholesterol is transported through the blood as lipoproteins: low-density lipoprotein (LDL) and high-density lipoprotein (HDL). An optimal amount of these proteins is necessary for proper body functioning.

CA125 is a protein found in most ovarian cancer cells and some normal tissues. A CA125 (Cancer Antigen 125) test is commonly advised to detect ovarian cancer by measuring the amount of CA125 protein in the blood. A deranged level of CA125 antigen may indicate the presence of certain cancers, primarily ovarian cancer or other non-cancerous conditions, including endometriosis, pelvic inflammatory disease, ovarian cysts, etc.

The CA15.3 test measures the amount of Cancer Antigen 15.3 in your blood. It is a protein that is often elevated in the blood of individuals with breast cancer. Therefore, CA15.3 is an important tool in monitoring the progress of breast cancer treatment and checking for recurrence.

To make a precise diagnosis, healthcare advisors recommend the CA15.3 test results in conjunction with other blood tests and diagnostic imaging tests. Individuals undergoing the CA15.3 test should discuss the results with their healthcare provider to gain a comprehensive understanding of their cancer status and make informed decisions about their treatment plan.

The Urine R/M (Urine Routine & Microscopy) test involves gross, chemical, and microscopic evaluation of the urine sample.

1. Gross examination: It involves visually inspecting the urine sample for color and appearance. Typically, the urine color ranges from colorless or pale yellow to deep amber, depending on the urine’s concentration. Things such as medications, supplements, and some foods such as beetroot can affect the color of your urine. However, unusual urine color can also be a sign of disease.

   In appearance, the urine sample may be clear or cloudy. A clear appearance is indicative of healthy urine. However, the presence of red blood cells, white blood cells, bacteria, etc., may result in cloudy urine, indicating conditions such as dehydration, UTIs, kidney stones, etc. Some other factors, such as sperm and skin cells, may also result in a cloudy appearance but are harmless.

2. Chemical examination: It examines the chemical nature of the urine sample using special test strips called dipsticks. These test strips are dipped into the urine sample and change color when they come in contact with specific substances. The degree of color change estimates the amount of the substance present. Some common things detected include protein, urine pH, ketones, glucose, specific gravity, blood, bilirubin, nitrites, and urobilinogen.

3. Microscopic examination: This involves the analysis of the urine sample under the microscope for pus cells, red blood cells, casts, crystals, bacteria, yeast and other constituents.

The BMI test measures body fat using a formula based on weight and height. Specifically, it calculates the ratio of an individual's weight in kilograms to the square of their height in meters (or weight in pounds to the square of height in inches, with a conversion factor). The resulting value is categorized into ranges that indicate whether an individual is underweight, normal weight, overweight, or obese.

The categories are as follows: underweight (BMI less than 18.5), normal weight (BMI between 18.5 and 24.9), overweight (BMI between 25 and 29.9), and obesity (BMI 30 or greater). Each category provides a general indication of body weight status and associated health risks. While BMI offers a convenient measure of body weight relative to height, it is best used in conjunction with other health assessments, such as body fat percentage, diet, and physical activity levels, to gain a comprehensive understanding of an individual’s health.

The Blood Urea Nitrogen test measures the levels of urea nitrogen in the blood. Blood urea is a waste product that is formed in the liver when you eat food and the protein is metabolized into amino acids. This process leads to the production of ammonia that is further converted into urea. Both ammonia and urea are nitrogenous compounds. Your liver releases urea into the blood which is then carried out to the kidneys. In the kidneys, urea is filtered from the blood and flushed out of the body via urine. This is a continuous process, so a small amount of urea nitrogen always remains in the blood.

In the case of a kidney or liver disease, there is a change in the amount of urea present in the blood. If your liver produces urea in an increased amount or if there is any problem in kidney functioning, there might be difficulty in filtering out the waste products from the blood, which can result in increased urea levels in the blood.

An HbA1c (Glycosylated Hemoglobin) test precisely measures the percentage of sugar-coated or glycated hemoglobin in your blood. The test results represent the proportion of hemoglobin in your blood that has been glycated. 

Hemoglobin, a vital protein found in red blood cells, is responsible for transporting oxygen throughout the body. Hemoglobin A is the most abundant form of hemoglobin, and when blood sugar levels increase, a higher proportion of hemoglobin A becomes glycated. As red blood cells have a lifespan of approximately 120 days, the sugar molecules remain attached to the hemoglobin for the duration of the cell's life. Consequently, the HbA1c test offers insight into your average blood sugar levels over the past 8 to 12 weeks.

Gamma-Glutamyl Transferase (GGT) is an enzyme found in various organs, with the highest concentration in the liver. Usually, this enzyme is present in low levels in the blood. However, when there is liver damage or disease, GGT is released into the bloodstream, causing an increase in GGT levels. In addition to the liver, GGT can also be elevated in conditions affecting the bile ducts or the pancreas. It is usually, the first liver enzyme to rise in the blood when there is any damage or obstruction in the bile duct, making it one of the most sensitive liver enzyme tests for detecting bile duct problems.

The Bilirubin Total examination quantifies the levels of total bilirubin in the body, encompassing both indirect (unconjugated) and direct (conjugated) bilirubin. Bilirubin, a yellowish waste substance, is primarily generated during the breakdown of aging red blood cells (RBCs) in the body. After their typical lifespan of 120 days, RBCs disintegrate in the liver, leading to the production of a substantial amount of bilirubin. It is crucial for this bilirubin to be eliminated from the body.

A CA19.9 test measures the level of a specific tumor marker called CA 19.9 in the blood, often associated with gastrointestinal cancers, particularly pancreatic cancer. This biomarker is produced by certain cells in the body, specifically epithelial cells. These cells are found in various tissues, including the gastrointestinal tract. Elevated levels of CA 19.9 are often associated with cancers that involve these tissues, such as pancreatic, colorectal, and gastric cancers. However, CA 19.9 can also be produced by non-cancerous conditions, impacting its specificity as a diagnostic marker. For a comprehensive evaluation, it is advised to consider other diagnostic tests in conjunction with a CA19.9 test and consult a doctor for a proper understanding of the test results.

An SGPT (Alanine Transaminase) test measures the amount of alanine transaminase (ALT) or SGPT enzyme in your blood. ALT is most abundantly found in the liver but is also present in smaller amounts in other organs like the kidneys, heart, and muscles. Its primary function is to convert food into energy. It also speeds up chemical reactions in the body. These chemical reactions include the production of bile and substances that help your blood clot, break down food and toxins, and fight off an infection.

Elevated levels of ALT in the blood may indicate liver damage or injury. When the liver cells are damaged, they release ALT into the bloodstream, causing an increase in ALT levels. Therefore, the SGPT/ALT test is primarily used to assess the liver's health and to detect liver-related problems such as hepatitis, fatty liver disease, cirrhosis, or other liver disorders.

An Alkaline Phosphatase (ALP) test measures the quantity of ALP enzyme present throughout the body. The main sources of this enzyme are the liver and bones. It exists in different forms depending on where it originates, such as liver ALP, bone ALP, and intestinal ALP. In the liver, it is found on the edges of the cells that join together to form bile ducts. 

ALP levels can be increased during pregnancy as it is found in the placenta of pregnant women. It is also higher in children because their bones are in the growth phase. ALP is often high during growth spurts (a short period when an individual experiences quick physical growth in height and body weight).

An SGOT (Aspartate Aminotransferase) test measures the levels of serum glutamic-oxaloacetic transaminase (SGOT), also known as aspartate aminotransferase (AST), an enzyme produced by the liver. SGOT is present in most body cells, most abundantly in the liver and heart. The primary function of this enzyme is to convert food into glycogen (a form of glucose), which is stored in the cells, primarily the liver. The body uses this glycogen to generate energy for various body functions.

The Carcino Embryonic Antigen test measures the level of a specific tumor marker called CEA In the blood, often associated with colon and rectal cancer. This biomarker is produced by certain tissues in the body, specifically gastrointestinal tissues, during fetal development. CEA levels drop to low or nonexistent levels after birth. Elevated levels of CEA in the blood may indicate the presence of certain cancers, particularly colorectal cancer. However, it's important to note that high CEA levels can also be linked to non-cancerous conditions.

For a comprehensive evaluation, it is advised to consider other diagnostic tests in conjunction with Carcino Embryonic Antigen test and consult a doctor for a proper understanding of the test results.

An Alpha Feto Protein, Tumour Marker test that measures the level of AFP (alpha-fetoprotein) in your blood sample. Alpha-fetoprotein is a protein produced by the liver of a developing baby (fetus). AFP circulates through the fetal and maternal blood. It is present during pregnancy but disappears rapidly after birth. However, elevated AFP levels in adults can be a sign of liver disease, liver cancer, or cancers of the testes or ovaries. An Alpha Feto Protein, Tumour Marker can also be used for assessing response to ongoing treatment and monitoring for cancer recurrence.

An HIV Combo (Antigen And Antibody) Test simultaneously detects HIV p24 antigen and antibodies associated with HIV 1 & 2. The HIV p24 antigen is the most abundant HIV protein. Its detection is used clinically to diagnose an HIV infection early on since HIV-specific antibodies are not detectable in a very recently acquired infection.

HIV-1 was discovered first and is the most widespread. HIV-2 is more than 55% genetically different from HIV-1. The transmission rate of HIV-2 is also slower than HIV-1. 

The blood pressure test measures the force of blood against the walls of the arteries as the heart pumps it throughout the body.

The results are expressed in millimeters of mercury (mmHg) and presented as a ratio, with systolic pressure listed first followed by diastolic pressure.  A normal blood pressure reading is generally around 120/80 mmHg. Readings higher or lower than this can signal potential health issues. Elevated systolic or diastolic readings might indicate conditions such as hypertension, which can lead to more serious health problems like heart disease or stroke if not managed properly. Conversely, abnormally low readings might suggest issues like poor heart function or dehydration. By assessing these pressures, the test helps evaluate heart health, diagnose potential cardiovascular issues, and guide appropriate treatment or lifestyle adjustments.

The Hepatitis C Virus (HCV) Antibody test determines the status of current hepatitis C virus (HCV) infection or past HCV infection that has been resolved. This test, therefore, helps your doctor to guide an appropriate treatment plan. HCV causes liver inflammation and is classified into acute hepatitis infection and chronic hepatitis infection.

• Acute hepatitis C: This occurs in the first six months after exposure to the hepatitis C virus. In the early stage of the infection, acute hepatitis C is mild and may cause no symptoms. For this reason, most people do not know they have this infection.
• Chronic hepatitis C: If your body is unable to fight off the virus, you can develop a chronic hepatitis C infection. Progression from acute to chronic hepatitis C is common, occurring in about 75-85% of patients. Detecting chronic hepatitis C at the early stage is important to prevent future complications, such as liver failure, and initiate effective treatment.

The Hepatitis C Virus (HCV) Antibody test is used for screening for anti-HCV antibodies. A reactive result needs to be confirmed by other more specific NAT/ nucleic acid tests before final diagnosis.