Cortisol, Serum (Morning Sample)

Also referred as

CORTISOL

Serum Cortisol

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The Cortisol, Serum (Morning Sample) is a blood test that measures the level of cortisol, a steroid hormone produced by the adrenal gland. It is typically done in the morning when cortisol levels are at their peak, helping diagnose conditions associated with adrenal gland functioning, such as Cushing’s syndrome or Addison’s disease.

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Understanding Cortisol, Serum (Morning Sample)

Cortisol is a vital hormone produced by the adrenal glands, two small glands that are located above the kidneys. It affects almost every organ and tissue in your body and plays various roles such as:

Control the balance of water and electrolytes
Reduce inflammation
Support your immune system
Handle stress (often called the “stress hormone”)
Maintain blood pressure and blood sugar levels

Abnormal cortisol levels may suggest problems with the adrenal glands, pituitary gland, or even the presence of a tumor that produces cortisol.

High cortisol levels may occur in conditions like Cushing’s syndrome, tumors of the adrenal or pituitary glands, and in some cases, due to tumors outside these glands, which make ACTH (a hormone that triggers cortisol production). Cortisol can also increase because of long-term steroid medication use, emotional or physical stress, low blood sugar (hypoglycemia), depression, and an overactive thyroid (hyperthyroidism). On the other hand, low cortisol levels can be seen in Addison’s disease, poor function of the adrenal glands (called adrenal insufficiency), or problems in the body’s process of making cortisol.

Cortisol levels in the body are controlled by ACTH, a hormone made by the pituitary gland. The release of ACTH is triggered by another hormone called corticotropin-releasing hormone (CRH), which is produced by the hypothalamus. This process works in a daily cycle. Cortisol levels are highest in the early morning (around 6 a.m. to 8 a.m.) and lowest late at night (around 11 p.m.).

The Cortisol, Serum (Morning Sample) test is usually done early in the morning because cortisol levels are highest at that time. This helps get the most accurate results and gives a clear picture of how well the adrenal glands are working. In people with Cushing’s syndrome, this rhythm is often disrupted, and cortisol levels may remain high even in the late evening. By measuring cortisol levels later in the day, doctors can distinguish between normal daily variation and the abnormal cortisol production seen in Cushing’s syndrome. However, these levels can also be affected by stress or other health conditions. Since cortisol follows the body’s daily (circadian) rhythm, your doctor may ask you to avoid vigorous exercise the day before the test, as it may affect the results.

Lab test results may vary across laboratories. Abnormal test results require an expert interpretation. Therefore, never try to self-medicate at home based solely on these results, and always consult a doctor for a proper understanding of the test results.

What does Cortisol, Serum (Morning Sample) measure?

The Cortisol, Serum (Morning Sample) test measures the levels of the cortisol hormone in the blood. Cortisol is a vital hormone produced by the adrenal glands in response to stress and plays an important role in various bodily functions such as metabolism, immune response, and the body’s stress response system.

This test is conducted during the morning because cortisol levels fluctuate throughout the day, typically peaking in the early morning and gradually declining throughout the day and then into the evening. By measuring the cortisol levels in the morning, the doctors can assess the body’s natural rhythm (circadian rhythm) and evaluate how well the adrenal glands and pituitary glands are working. Deranged cortisol levels can indicate conditions such as adrenal insufficiency (Addison’s disease), cortisol excess (Cushing’s syndrome), or other disorders affecting these glands. Additionally, this test helps diagnose and monitor conditions related to stress, sleep disorders, metabolism, and overall hormone balance.

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Test Information is reviewed by: Dr. Swati Gupta (Anand), MD Lab Medicine, MBBS and written by Dr. Lipika Khurana, PGDHHM, BDS . The test details are for information purpose only. Consult a doctor before taking any test. Last updated on: 8th Oct 2026

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Cortisol, Serum (Morning Sample) test price for other cities

Price in	Bangalore	Rs. 849
Price in	Mumbai	Rs. 809
Price in	New Delhi	Rs. 659
Price in	Hyderabad	Rs. 649
Price in	Pune	Rs. 679

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FAQs related to Cortisol, Serum (Morning Sample)

Why is a cortisol blood test done in the early morning?

Your doctor will likely have you do the test early in the morning. This is important because cortisol level varies throughout the day.

Does a cortisol blood test require fasting?

You do not need to fast for this test. Cortisol levels vary throughout the day but are usually highest in the morning. Your doctor will usually request that the test be done in the morning.

How is the Cortisol, Serum (Morning Sample) test done?

For this test, a blood sample is collected from a vein in your arm. The sample is then analyzed in a laboratory to determine the levels of the cortisol hormone.

What do the abnormal results indicate?

Abnormal cortisol levels can indicate various conditions. High levels may indicate Cushing’s syndrome, stress, etc., while low levels may indicate Addison’s disease, pituitary gland problems, etc.

Are there any risks associated with the cortisol test?

This test is safe with minimal or no risk. However, you may feel slight discomfort or bruising at the blood draw site, which is not bothersome and usually self-limiting.

What other tests may be done along with cortisol?

Your doctor may also ask for an ACTH (adrenocorticotropic hormone) test, electrolyte levels, or a 24-hour urine cortisol test to get a better picture of your adrenal and pituitary gland function.

Why is cortisol called the “stress hormone”?

Cortisol levels increase when you are stressed. It helps your body cope by increasing energy, improving alertness, and reducing inflammation.

How does Tata 1mg ensure accurate lab test results?

Tata 1mg ensures accurate lab test results through certified laboratories. Tata 1mg uses advanced technology and adheres to stringent quality control measures. This commitment to high standards guarantees reliable results.

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