HRDTrack Test in Kolkata
Understanding HRDTrack Test in Kolkata
What is HRDTrack Test in Kolkata?
The HRDTrack test is a crucial diagnostic tool used in the field of oncology to assess the status of Homologous Recombination Deficiency (HRD) in patients. HRD is a biological phenomenon where cells fail to effectively repair DNA double-strand breaks, leading to an increased occurrence of genomic alterations. While the HR pathway, which includes genes like BRCA1/2, is essential for accurate DNA repair, HRD can occur even without mutations in these genes. Remarkably, over 50% of high-grade serous ovarian cancer patients exhibit HR deficiency, with only approximately 20% of them carrying BRCA1/2 mutations.
This test determines HRD status by analyzing genome-wide aberrations resulting from defects in the Homologous Recombination Repair (HRR) pathway and pathogenic mutations in BRCA1 and BRCA2 genes. It begins by extracting DNA from FFPE tissue samples and preparing targeted Next-generation sequencing libraries with Unique Molecular Identifiers (UMIs). These enriched libraries are then sequenced using Illumina sequencing platforms, such as Novaseq, following rigorous quality control checks. The resulting sequencing data is used to identify mutations in BRCA1 and BRCA2 genes and compute the HRD status.
The results of this test serve as a valuable aid in determining the eligibility of patients for specific treatments, such as Poly-ADP Ribose inhibitors, by providing critical insights into HRD status and genomic stability. This diagnostic tool plays a pivotal role in personalized medicine, helping healthcare professionals make informed decisions about treatment strategies for cancer patients.
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