HLA - B27 Detection by Flowcytometry

Human leukocyte antigens (HLAs) are proteins that help the body's immune system tell the difference between its own cells and foreign invaders. They are made from instructions by inherited genes. Individuals with the HLA B-27 gene are more likely to develop autoimmune diseases such as ankylosing spondylitis, juvenile rheumatoid arthritis, rheumatoid arthritis type II, lupus erythematosus, Crohn's disease, etc. Your doctor may suggest an HLA - B27 Detection by Flowcytometry test if you experience signs and symptoms such as stiffness and chronic pain in the back, neck, or chest, inflammation in eyes, urethra, and joints, the appearance of skin lesions, recurring fever, abdominal pain, or digestive problems indicative of an autoimmune disorder. 

An HLA - B27 Detection by Flowcytometry test is also used during an organ transplant to match donated tissue with a person's tissue who is getting a transplant to minimize the risk of organ rejection and improve the success of the transplant. 

HLA B-27 gene detection can be done by two methods - flow cytometry and Polymerase chain reaction (PCR). An HLA - B27 Detection by Flowcytometry test identifies the expression of the HLA B-27 gene on WBC surfaces whereas the PCR method directly identifies the HLA B-27 gene within the genetic material. Both these tests help diagnose autoimmune diseases but the choice between these methods often depends on the clinical context and the specific disease being investigated. 

No special preparation is required for an HLA - B27 Detection by Flowcytometry test. Lab test results may vary across different laboratories. Abnormal test results require an expert interpretation, therefore, never try to self-medicate at home based solely on these results, and always consult a doctor for proper understanding of the test results. Discuss your symptoms and medical history to help the doctor correlate your clinical and laboratory findings.