Test Detail
Overview
Interpreting Results
FAQ's

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Written by
Dr. Shreya Gupta
BDS, MDS - Oral and Maxillofacial Pathology
Reviewed by
Dr. Ashish Ranjan
MBBS, MD (Pharmacology)
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HIV 1 And 2 Antibody - ELISA

HIV 1 And 2 Antibody - ELISA
You need to provide
Blood
This test is for
Male, Female
Test Preparation
  1. No special preparation required

Overview


What is HIV 1 And 2 Antibody - ELISA?

This test is done to confirm the HIV-1 and HIV-2 antibodies present in the blood. It also helps in differentiating HIV-1 and HIV-2 antibodies in specimens of serum which show reactive results with third and fourth generation HIV serologic assays. This test is not a screening test to detect HIV infection.

Why is HIV 1 And 2 Antibody - ELISA done?

  • To screen for and diagnose HIV infection 

  • If you are pregnant or planning to get pregnant 

  • If you are diagnosed with Hepatitis B, C, Tuberculosis, or any other sexually transmitted disease

  • Annual screening is advised for those who are at high risk for HIV infection like having an HIV positive sex partner, multiple sexual partners, homosexual people, and sharing needles

  • To differentiate HIV-1 and HIV-2 antibodies in specimens of serum that show reactive results with third and fourth generation HIV serologic assays



What does HIV 1 And 2 Antibody - ELISA Measure?

Acquired Immunodeficiency Syndrome (AIDS) is caused by the Human Immunodeficiency Virus (HIV). HIV Virus is of two types: HIV-1 and HIV-2. HIV-1 is found in AIDS patients, AIDS-related complex patients, and those persons who are at high risk of getting affected by AIDS. HIV virus can be transmitted by sexual contact, exposure to blood or blood products of AIDS-infected patients, or transfer of infection from AIDS-infected mother to the fetus. Most of the cases of HIV-2 infected patients are seen in West Africa, and it is detected in patients who had sexual contact with people of that geographic region. These viruses have the same morphology, overall genomic structure, and ability to cause infection. 

When the patient gets infected with the virus, the virus attacks the immune system called CD4 and combines with antibodies to use them for making a large number of copies inside the body of the patient by replication. During the first few weeks of infection, the amount of virus present and the level of p24 antigen in the blood becomes very high. After another 2-8 weeks, these increased levels start to fall as the body of the infected patient starts producing antibodies against the antigen of the virus. At this time, the HIV virus antibody can be detected in the patient’s blood via tests. The tests should be performed at this brief time because after some time both the levels of antigen and antibody in the blood falls. This will make the detection of virus infection difficult as the initial infection may have been resolved and you may not get a positive result against the infection.

The HIV antibody tests do not detect an HIV infection soon after exposure, before the development of antibodies. Most people produce detectable levels of antibody 3 to 12 weeks after exposure. If someone is screened with an HIV antibody test too soon, the result may be negative despite the fact that the person is infected. For those who are at increased risk of HIV infection, it is important to get this screening test done frequently to check for possible exposure to the virus.


This test is done by ELISA method which is also known as EIA for Enzyme Immunoassay. This test is done to analyze certain proteins which are produced by the body in response to HIV infection. 

The blood sample is added to a cassette which contains the HIV antigen. If the patient’s blood contains HIV antibodies, they will bind with the antigen and there will be changes in the content of the cassette.



Interpreting HIV 1 And 2 Antibody - ELISA results


Interpretations

  • A negative HIV antibody test usually indicates that a person does not have HIV infection. However, it only means that there is no evidence of disease at the time of the test.
  • If someone tests positive on both the initial screening and supplemental testing, it is considered as a definite case of infection with HIV.



Answers to Patient Concerns & Frequently Asked Questions (FAQs) about HIV 1 And 2 Antibody - ELISA


Frequently Asked Questions about HIV 1 And 2 Antibody - ELISA

Q. Which screening tests are advised by the doctor for patients under the risk of HIV?
There are different types of tests available for HIV testing: Combination HIV antibody and HIV antigen test, HIV antibody testing, and p24 antigen testing
Q. Is HIV infection curable?
HIV infection is not curable but if diagnosed early, proper treatment can be started. HIV infection is treated with antiretroviral therapy (ART). This therapy helps in reducing the levels of virus in the body which in turn will improve the patient’s health.
Q. How is the blood sample taken?
The healthcare provider takes a blood sample from the arm. The site from where the blood is to be withdrawn is cleaned with a swab of rubbing alcohol. This is then followed by inserting a small needle which has a tube attached to it for collecting blood. Once the sufficient blood for analysis is withdrawn, the needle is removed. The site is then covered with a gauze pad.
Q. Is there any risk associated with the withdrawal of blood sample procedure?
As such there is no risk but in few cases, bruising, bleeding, and infection at the puncture site can be seen. In very few cases, there can be swelling of the vein after the blood is withdrawn.
Q. What are the symptoms of HIV infection?
The symptoms of HIV infection are similar to that of influenza and other viral infections such as fever, sore throat, muscle, and joint pain. It is possible that the symptoms may go unidentified for years. In order to avoid such a condition, the best way is to get screened for HIV infections.
Q. Is there any special preparation required for the test?
No, there is no special preparation required for the test.
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