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Culture Urine
Urine C/S
Overview
Interpretations
FAQ's
Culture Urine

Overview of Culture Urine

What is Culture Urine?

The waste products are excreted out of the blood by the kidneys in the form of a light straw colored liquid called urine. The path taken by the urine from the kidneys to the outside is called the urinary tract. The urinary tract gets sometimes infected by microorganisms. The Urine Culture Test is performed to detect and diagnose a microbial infection of the urinary tract.

Sample Type

The sample type collected for Culture Urine is: Urine

Preparation for Culture Urine

  • Collect the Urine Sample in a sterile container available from the lab or any pharmacy. Allow a small stream of urine to flow by and then carefully hold the cup under urine. Make sure the cup doesn’t come in contact with your skin.

Why Get Tested for Culture Urine?

The Urine Culture Test is performed:

·       To detect and diagnose a urinary tract infection (UTI) by bacteria or yeast in the presence of symptoms

·         To screen for urinary tract infections in the first trimester of pregnancy

·         As a part of routine health checkups



Understand more about Culture Urine

Metabolic processes in the body produce products called metabolites which are present in the blood. Some of them are utilized by cells, while others are wastes which are filtered out of the blood by the kidneys in a solution called urine. Urine produced by the kidneys pass through two tubes called ureters into the urinary bladder where it is stored until the bladder is full. Once full, the urinary bladder releases the urine through another tube called urethra to the outside of the body. The pathway followed by urine from the kidneys to the outside is called the urinary tract. The urinary tract may be infected by some bacteria or yeast causing various conditions. Urine is normally sterile, but if urinary tract infection occurs, the pathogenic microorganism may be found in urine. A urine sample is collected and cultured to detect such an infection and to identify the microorganism causing it.

The collected urine sample is placed on an agar medium (nutrient solution mixed with agarose gel) and incubated at body temperature for 24 to 48 hours. This allows growth of the microorganisms in the sample if any. Growing microorganisms form different types of colonies on the agar which are studied further in a laboratory to determine the exact microbe causing the UTI.

Once the pathogen is identified, antibiotic susceptibility test is performed to guide treatment for the UTI being tested for. This may be done in one of two ways. The conventional method involves the use of small filter paper discs with antibiotic of known concentration and placing them on agar plate where the pathogen is cultured. The antibiotic creates a small area around the discs where the microorganisms do not form colonies. The radius of this zone is measured to estimate the efficacy and strength of that antibiotic in treating the UTI. The other method involves an automated machine to detect the sensitivity pattern to find out which antibiotic can be used to treat the infection.


What Results of Culture Urine mean?

Interpretations

Negative result: No colonies to less than 1,00,000 CFU/ml (Colony Forming Units)

Positive result:

·         Usually more than 1,00,000 CFU/ml

·         Patient undergoing antibiotic treatment: More than 1,000 CFU/ml

·         For gram-positive bacteria like Staphylococcus aureus: Single colony

The negative result indicates no infection of the urinary tract.

The positive result indicates an infection of the urinary tract. The positive result is accompanied by an antibiotic susceptibility test.

In an uncontaminated sample, only a single type of colony is found and indicates a clinically significant positive result. However, if multiple colonies of different types are found, a sample contamination is indicated and the sample may need to be collected again.


Patient Concerns about Culture Urine

Frequently Asked Questions about Culture Urine

Q. Is there any preparation required before the Urine culture test?
It may be advised to collect an early morning urine sample (during the first urination after waking up). Inform the doctor of any medications you may be taking. No other specific preparations are usually required before the Urine Culture Test.
Q. How is Urine culture test performed?
The test is performed on the urine sample. It is essential to prevent contamination of the sample with microorganisms naturally present on the skin. During urine collection, women should spread the labia (outer lips of the vagina) while men should retract or pull back the foreskin (fold of skin covering the glans or head of the penis). Start urinating normally and let some urine flow before collecting 20 to 30 ml urine the sterilized container available from the lab or any pharmacy. After collecting the sample, finish urinating into the toilet or urinal. This process is called midstream clean catch method. The urine sample may also be collected using a catheter inserted through the urethra directly into the urinary bladder. Sample collected through a catheter usually has no contamination.
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