Vitamin D, B12 & Calcium

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The Vitamin D, B12 & Calcium test measures the levels of vitamin D (25-Hydroxy), vitamin B12, and calcium in the blood. These tests are essential for evaluating bone strength, nerve function, and overall health. These tests help detect deficiencies or excesses that could lead to health problems.

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Understanding Vitamin D, B12 & Calcium

The Vitamin D, B12 & Calcium test provides vital information about the overall health. Vitamin D (25-Hydroxy) is important for calcium absorption and bone strength while also supporting immune function and showing an association with mood regulation. Vitamin B12 plays an essential role in red blood cell formation, nerve function, and energy production. Calcium is a key mineral for strong bones and teeth, as well as for proper muscle and heart function. Together, these tests help identify deficiencies that could lead to conditions like osteoporosis, fatigue, nerve damage, or muscle weakness, ensuring timely intervention and better health outcomes.

Deficiencies of these nutrients can lead to symptoms like fatigue, muscle weakness, bone pain, numbness, or poor immune function. On the other hand, excessive levels can also cause problems such as kidney stones, nerve damage (excess vitamin B12), etc. The Vitamin D, B12 & Calcium profile helps detect imbalances early, ensuring proper management and preventing complications. It is especially important for individuals with bone health concerns, dietary restrictions, or frequent symptoms like fatigue, muscle weakness, etc.

Overnight fasting is preferred for the Vitamin D, B12 & Calcium test but is not mandatory. Inform your doctor about all the medications or supplements that you may be taking, as these may affect the test results, potentially requiring dosage adjustments or temporary discontinuation before the test.

Lab test results may vary across laboratories. Do not self-medicate at home solely based on these results, and always consult a doctor for a proper understanding of the test results. The test results will help them assess your health status, make recommendations for lifestyle modifications such as diet and exercise, prescribe medication if required to manage your condition, and formulate your overall treatment plan.

What does Vitamin D, B12 & Calcium measure?

Contains 3 tests

The Vitamin D, B12 & Calcium test measures the levels of vitamin D (25-Hydroxy), vitamin B12, and calcium in the blood. These are the important nutrients that are essential for overall health. Vitamin D (25-Hydroxy) is crucial for bone health and immune function. Vitamin B12 is necessary for energy production, nerve health, and red blood cell formation. Calcium is vital for strong bones, muscle function, and heart health. This profile helps detect imbalances, guiding appropriate treatments or the need for supplementation.

Serum Calcium

The Serum Calcium test measures the calcium level in your blood. Most of your body’s calcium (about 99%) is stored in bones and teeth to keep them strong. The rest (1%) circulates in the blood and helps your muscles move, nerves work, blood to clot, and heart to keep a steady rhythm.

Calcium in blood comes in two forms:

Bound calcium, attached to proteins like albumin
Ionized (free) calcium, which is active and helps many body functions

This test measures total calcium (both bound and ionized). This test cannot show if you have enough calcium in your diet. It also cannot detect bone diseases like osteoporosis. The body keeps blood calcium levels steady. It does this by taking calcium from bones during mild shortages.

Know more about Serum Calcium

Vitamin B12

The Vitamin B12 test measures the amount of vitamin B12 in your blood. Vitamin B12 is important for a healthy nervous system, making red blood cells, and helping in DNA formation. Low B12 levels are common in older adults, children, vegans or vegetarians, people with diabetes, those who had gastric bypass surgery, breastfeeding women, and people with conditions like Crohn’s disease that affect vitamin absorption. High B12 levels can occur in liver diseases, certain blood disorders, or from taking vitamin supplements. Usually, high levels from supplements are not harmful, as extra B12 is removed through urine.

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Vitamin D (25-Hydroxy)

The Vitamin D (25-Hydroxy) test measures the levels of vitamin D in the body. Vitamin D is an essential nutrient that can be synthesized in the body upon healthy exposure to sunlight or absorbed from dietary sources. It exists in two forms: vitamin D2 (ergocalciferol) and vitamin D3 (cholecalciferol). Vitamin D2 is present in plants, such as yeast or mushrooms, and is available as a supplement in fortified foods. Vitamin D3 is found in foods like cheese, mushrooms, egg yolks, and fatty fish.

Both forms of vitamin D need to be converted in the liver and kidneys before the body can use them effectively. This test shows the total level of vitamin D (D2 + D3) circulating in the body and guides your doctor in recommending the right treatment or lifestyle changes if needed.

Know more about Vitamin D (25-Hydroxy)

Test Information is reviewed by: Dr. Swati Gupta (Anand), MBBS, MD Lab Medicine and written by Dr. Lipika Khurana, BDS, PGDHHM . The test details are for information purpose only. Consult a doctor before taking any test. Last updated on: 30th Aug 2026

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Vitamin D, B12 & Calcium test price for other cities

Price in	Hyderabad	Rs. 829
Price in	Bangalore	Rs. 1329
Price in	Pune	Rs. 1329
Price in	New Delhi	Rs. 1309
Price in	Mumbai	Rs. 1329

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FAQs related to Vitamin D, B12 & Calcium

Why should I take the Vitamin D, B12 & Calcium test?

This test helps detect deficiencies or excesses in these essential nutrients, which can impact bones, energy levels, immune function, and nerve health.

What happens if I have a vitamin B12 deficiency?

Vitamin B12 deficiency can cause tiredness, weakness, tingling in the hands and feet, poor memory, and anemia. If untreated, it may lead to nerve damage.

What happens if I don’t have enough vitamin D?

Vitamin D deficiency can cause bone pain, muscle weakness, and fatigue. In children, it may cause rickets (soft bones). In adults, it can lead to osteomalacia and increase the risk of osteoporosis.

Can I prevent these deficiencies?

Yes, eating a balanced diet and getting regular sunlight can help prevent these deficiencies. Taking supplements only if advised by your doctor can also help prevent these nutritional deficiencies.

What are the signs of low calcium levels?

Calcium deficiency can cause muscle cramps, numbness or tingling, weak nails, tooth problems, and weak bones.

Are there any risks associated with the Vitamin D, B12 & Calcium test?

No, usually, there are no risks associated with this test. The blood sample is withdrawn with the help of a needle, and the process is fairly fast and relatively painless. Very rarely, you may experience pain, bleeding, hematoma (blood collection under the skin), bruising, or infection at the site of the needle prick. Consult the doctor if you experience any of these.

Is fasting required for the Vitamin D, B12 & Calcium test?

Fasting is preferred for this test, but it is not mandatory.

How is the Vitamin D, B12 & Calcium test performed?

A phlebotomist will clean your skin using an antiseptic alcohol cotton swab or wipe and take a blood sample from your vein using a needle. The blood sample will be stored safely and transported to the laboratory for analysis.

How does Tata 1mg ensure accurate lab test results?

Tata 1mg ensures accurate lab test results through certified laboratories that use advanced technology and adhere to stringent quality control measures. This commitment to high standards guarantees reliable results.

How does Tata 1mg ensure accurate lab test results?

Tata 1mg ensures accurate lab test results through certified laboratories. Tata 1mg uses advanced technology and adheres to stringent quality control measures. This commitment to high standards guarantees reliable results.

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