Overview of Hemoglobin (Hb) Electrophoresis by HPLC
What is Hemoglobin (Hb) Electrophoresis by HPLC?
This assay is useful in the diagnosis of disorders associated with abnormal forms of hemoglobin like sickle cell anemia, thalassemia.
Why is Hemoglobin (Hb) Electrophoresis by HPLC done?
- If you have signs or symptoms of hemolytic anemia like jaundice, fatigue, pale skin or splenomegaly
- If your complete blood count and peripheral smear indicate that you may have an abnormal form of hemoglobin
- It is commonly done as a part of newborn screening
- It may used for prenatal screening if a parent is at high risk
Preparation for Hemoglobin (Hb) Electrophoresis by HPLC
- No special preparation required
Sample Type for Hemoglobin (Hb) Electrophoresis by HPLC
The sample type collected for Hemoglobin (Hb) Electrophoresis by HPLC is: Blood
Interpreting Hemoglobin (Hb) Electrophoresis by HPLC results
All the results should be carefully interpreted in correlation with the patient's signs and symptoms and other laboratory findings.
- Presence of high level of sickle cell hemoglobin (HbS ) on HPLC denotes either sickle cell trait or sickle cell anemia
- Increased levels of fetal hemoglobin (HbF) on HPLC is associated with beta thalassemia major. Sometimes, it can also be seen in sickle cell anemia
- High HbA2 levels are mostly associated with beta thalassemia minor or trait. Recent studies say that HbA2 level between 4 to 9% is diagnostic of beta thalassemia trait
- Other less common hemoglobins which can be detected are hemoglobin D, J, G, M etc.