Vitamin D Profile (D2, D3 & D Total)
Understanding Vitamin D Profile (D2, D3 & D Total)
What is Vitamin D Profile (D2, D3 & D Total)?
A Vitamin D Profile (D2, D3 & D Total) is a blood test that measures the levels of various forms of vitamin D in the body. This test is a valuable indicator of osteoporosis (bone weakness), rickets (bone malformation), and osteomalacia (bone softening). It can also be used to diagnose/monitor problems with the functioning of the parathyroid gland since the parathyroid hormone (PTH) is essential for vitamin D metabolism.
Vitamin D is a fat-soluble essential nutrient the body produces when the skin is exposed to sunlight. It exists in two primary forms: vitamin D2 (ergocalciferol), which is obtained from dietary sources like fortified foods and supplements, and vitamin D3 (cholecalciferol), which is produced in the skin when exposed to sunlight. Both forms of vitamin D are converted into an active hormone called calcitriol in the liver and kidneys. This hormone regulates calcium and phosphorus absorption, immune function, bone mineralization, and other physiological processes.
As per a study by NCBI (National Center for Biotechnology Information), vitamin D deficiency is highly prevalent across all age groups in India. Low vitamin D levels can lead to many disorders, such as an increased risk of fractures, muscle weakness and lethargy, dysfunction of the immune system, and an increased risk of chronic diseases like diabetes. A Vitamin D Profile (D2, D3 & D Total) can help determine its deficiency, and sometimes it may also help determine if you have too much vitamin D in your body (most likely from excessive supplements). Your doctor may suggest this test if you show symptoms suggestive of vitamin D deficiency, such as bone deformities, immune system dysfunction, muscle cramps and weakness, dental abnormalities, bone pain, fatigue, loss of appetite, nausea, and frequent urination.
Usually, no special preparation is required for this test; you may consume a regular diet before taking the test. However, it is generally recommended to stop taking vitamin D supplements before this test, as they may affect the accuracy of the test results. Also, let your doctor know about all your other medications, as some can affect the test results.
Test result ranges are approximate and may differ slightly between labs depending on the methodology and laboratory guidelines. Talk to your doctor about your specific test results. The test results will help them determine your medical condition, make recommendations for lifestyle modifications such as diet and exercise, prescribe medication if required to manage your condition and formulate your overall treatment plan.
What is Vitamin D Profile (D2, D3 & D Total) used for?
A Vitamin D Profile (D2, D3 & D Total) test can be done:
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To determine deficient, insufficient, or toxic vitamin D levels in the body.
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For routine checkups in individuals with vitamin D deficiency risk factors, including obesity, limited sun exposure, weight loss surgery, digestive disorders like Crohn’s disease, kidney or liver disease, and certain medicines like phenytoin.
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In individuals with vitamin D deficiency symptoms, such as bone deformities, immune system dysfunction, muscle cramps, and dental abnormalities.
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To monitor the adequacy of treatment in individuals who are receiving vitamin D supplements.
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To diagnose/monitor problems with the functioning of the parathyroid gland that affects vitamin D metabolism.
What does Vitamin D Profile (D2, D3 & D Total) measure?
Contains 3 testsA Vitamin D Profile (D2, D3 & D Total) measures all forms of vitamin D, including vitamin D3, vitamin D2, and vitamin D (Total). Vitamin D is an essential nutrient that can be synthesized in the body upon healthy exposure to sunlight or absorbed from dietary sources. It majorly exists in the form of vitamin D2 (ergocalciferol) and vitamin D3 (cholecalciferol). Vitamin D2 (ergocalciferol) is largely human-made and can be obtained from plant sources and fortified foods. In contrast, the body produces vitamin D3 (cholecalciferol) when your skin is exposed to sunlight. It can also be absorbed by eating animal-sourced foods.
Both forms of vitamin D (D2 and D3) need to undergo some chemical changes before being available for use in the body. They are absorbed equally well from your small intestine, metabolized in the liver or kidneys, and converted to measurable substances called 25-hydroxyvitamin D and 1,25-dihydroxyvitamin D. Vitamin D Total is the summation of both vitamins.
Vitamin D3
A Vitamin D3 test measures the amount of vitamin D3, also known as cholecalciferol, a type of vitamin D. Vitamin D3 is naturally found in animal-derived products like milk and may be consumed in certain fortified foods or dietary supplements. It is directly synthesized in the skin when it is exposed to the sun. A Vitamin D3 test is used to determine whether you have enough vitamin D3 in your body to support normal bodily functions.
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Vitamin D Total
A Vitamin D Total test measures the level of vitamin D2 and vitamin D3 in your body. This test is an appropriate indicator of the total Vitamin D storage in the body. A vitamin D total test is also recommended in case of abnormal calcium, phosphorus, and parathyroid hormone levels. Individuals with abnormal vitamin D levels become prone to heart diseases, high blood pressure, immune system disorders, different types of cancers (prostate, breast, and colon), and various infectious diseases like influenza, tuberculosis, sepsis, etc. All these reasons make it more important for individuals to keep a check on their vitamin D levels.
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Vitamin D2
A Vitamin D2 test measures the amount of vitamin D2, also known as ergocalciferol, which is a type of vitamin D. It is usually found in plant-based sources such as mushrooms and in some fortified foods. The Vitamin D2 test is ordered to determine if a deficiency, insufficiency, or abnormally high level of vitamin D is present in the body, or to monitor ongoing treatment for a previously diagnosed deficiency.
Know more about Vitamin D2
Interpreting Vitamin D Profile (D2, D3 & D Total) results
Interpretations
Vitamin D level below the normal reference range indicates vitamin D deficiency. This can be because of less sun exposure, dietary lack, or decreased absorption from the intestine.
Vitamin D level above the normal reference range indicates vitamin D intoxication. This is usually due to excess supplementation of the vitamin.
Range:
The normal range of vitamin D Total is measured as nanograms per milliliter (ng/mL) or nmol/L and can vary from lab to lab.
Level |
Reference range (ng/mL) |
Deficiency |
<20 ng/mL |
Insufficiency |
20-30 ng/mL |
Sufficiency |
30-100 ng/mL |
Toxicity |
>100 ng/mL |
Note:
- The assay measures vitamin D2 (Ergocalciferol) and vitamin D3 (Cholecalciferol) metabolites of vitamin D.
- There can be seasonal variation in 25 (OH) vitamin D levels, with values being 40-50% lower in winter than in summer. It is also influenced by sunlight, latitude, skin pigmentation, sunscreen use, and hepatic function.
- 25 (OH) vitamin D levels can vary with age
- 25 (OH) vitamin D level is increased in pregnancy.