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Understanding the Test
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Interpreting Results
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References
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Content created by
Written by
Dr. Nitika Vashisht
MD Pathology, MBBS
Reviewed by
Dr. Ashish Ranjan
MBBS, MD (Pharmacology)
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Urine C/S (Urine Culture and Sensitivity)

Also known as Urine culture test
769810 5% Off
You need to provide
Urine
This test is for
Male, Female
Test Preparation
  1. The urine sample must preferably be the first-morning midstream urine (part of urine that comes after the first and before the last stream). Please avoid urinating for at least 4 hours before collecting the sample to ensure accurate results. Also avoid drinking excessive fluids before collecting your urine sample, as it may dilute the urine and affect the results.
  2. Collect the urine sample in a sealed and sterile screw-capped container provided by our sample collection professional. Ensure that the urethral area (from where the urine is passed) is clean & the container doesn't come in contact with your skin. Women are advised not to give the sample during the menstrual period unless prescribed.
  3. If you are on any antibiotic(s), please provide clinical history, prescription, and/or specify the name of the antibiotic(s) as it may affect your test results.

Understanding Urine C/S (Urine Culture and Sensitivity)


What is Urine C/S (Urine Culture and Sensitivity)?

The Urine C/S (Urine Culture and Sensitivity) test helps detect harmful bacteria and yeast (a few Candida spp.) in urine that may be causing a urinary tract infection (UTI). Additionally, the sensitivity test helps identify the antibiotics most effective against those particular bacteria. It is also used to determine if there is resistance to any antibiotics.

Urine is the body’s liquid waste made when the kidneys filter out toxins from the blood. It is made up of water, salt, electrolytes such as sodium and potassium, and some metabolic end products. The pathway followed by urine from the kidneys to the outside is called the urinary tract. Sometimes, the urinary tract may get infected by some microorganisms, causing a UTI.

The Urine C/S (Urine Culture and Sensitivity) test is usually done when you experience symptoms of UTI, such as:

  • Burning sensation while passing urine
  • Back pain
  • Lower abdominal pain
  • Urinating more often than usual
  • Painful or frequent urination
  • Blood in the urine, etc., for appx. 3-4 days and does not subside

This test helps identify infection-causing microorganisms. If the results are positive, the sensitivity test helps determine which drugs (or antibiotics) are likely to be most effective for treating the infection. On the other hand, a negative urine culture indicates no signs of microbial infection; hence, it will not show sensitivity to various antibiotics.

Before the test, avoid urinating for at least 4 hours before collecting the sample to ensure accurate results. Also, avoid drinking excessive fluids before collecting the urine sample, as it may dilute the urine and affect the results. The doctor may ask you to stop taking certain medications, like antibiotics and supplements, before a Urine C/S (Urine Culture and Sensitivity) test, as they may affect your test results. A midstream urine sample should be collected into a sterile container provided by the sample collection professional. Women are advised not to give the sample during the menstrual period unless prescribed, as that can interfere with certain test results.

Test result ranges are approximate and may differ slightly between different labs depending on the methodology and laboratory guidelines. Talk to your doctor about your specific test results. Narrate your complete medical history to help the doctor correlate your clinical and laboratory findings. The test results will help them determine your medical condition and formulate your overall treatment plan.

Lifestyle Tips to Prevent Urinary Tract Infection (UTI)

🥤Hydration: Keep it up! Stay hydrated to help flush out bacteria and reduce the likelihood of urinary tract infections (UTIs).

🥙Eat (Diet): Cranberry compounds may help prevent urinary tract infections (UTIs)

🚴Activity (Exercise): Pelvic floor exercises, also known as Kegel exercises, help strengthen the muscles that help empty the bladder.

Lifestyle (Hygiene): After urination or bowel movements, wipe from back to front to keep gut bacteria from getting into the urethra. 

👨‍⚕️Talk to Your Doctor (Consult): If you have persistent UTI symptoms or are at high risk, consult your doctor about taking a urine culture test.

💊Habits (Antibiotics): Do not self-medicate. Your doctor may prescribe antibiotics to treat UTI.

What is Urine C/S (Urine Culture and Sensitivity) used for?

The Urine C/S (Urine Culture and Sensitivity) test can be done:

A. In Symptomatic Individuals

  • Pain or burning while urinating
  • Frequent urination
  • Feeling the need to urinate despite having an empty bladder
  • Bloody urine
  • Pressure or cramping in the groin or lower abdomen
  • Fever
  • Chills

B. In Individuals With Risk Factors

  • A previous UTI
  • Recent sexual activity

C. In Individuals With Lifestyle-Related Risks

  • Poor personal hygiene

D. In Special Populations

  • Pregnancy
  • Age (older adults and young children are more likely to get UTIs)

E. In Individuals With Anatomical Changes 

  • Structural problems in the urinary tract, such as an enlarged prostate

What does Urine C/S (Urine Culture and Sensitivity) measure?

The Urine C/S (Urine Culture and Sensitivity) test helps identify the bacteria or yeast (few candida spp.) causing UTI; the most common bacteria responsible for UTI is E.coli. Once the pathogen (harmful microorganism) is identified, the antibiotic susceptibility test is done to formulate a treatment plan for the infection.

Interpreting Urine C/S (Urine Culture and Sensitivity) results


Interpretations

Negative result: No colonies to less than 1,00,000 CFU/ml (Colony Forming Units)

Positive result:

·         Usually more than 1,00,000 CFU/ml

·         Patient undergoing antibiotic treatment: More than 1,000 CFU/ml

·         For gram-positive bacteria like Staphylococcus aureus: Single colony

The negative result indicates no infection of the urinary tract.

The positive result indicates an infection of the urinary tract. An antibiotic susceptibility test accompanies the positive result.

Only a single colony type is found in an uncontaminated sample, indicating a clinically significant positive result. However, if multiple colonies of different types are found, sample contamination is indicated, and the sample may need to be collected again.

Answers to Patient Concerns & Frequently Asked Questions (FAQs) about Urine C/S (Urine Culture and Sensitivity)


Frequently Asked Questions about Urine C/S (Urine Culture and Sensitivity)

Q. What are the common UTI-causing microorganisms?

Common microorganisms that may cause a UTI are Escherichia coli (E.coli), Klebsiella spp., Proteus spp., Pseudomonas aeruginosa, Staphylococcus aureus, Enterococcus spp., Acinetobacter spp., Staphylococcus saprophyticus, and Candida spp. Sometimes a person may also get UTI due to less common organisms like Enterobacter spp, Providencia spp, Citrobacter spp, etc.

Q. What is a UTI?

A urinary tract infection (UTI) is an infection that affects any part of the urinary system, which includes the kidneys, bladder, ureters, and urethra. Most infections involve the lower urinary tract — the bladder and the urethra.

Q. What is the difference between sensitive and resistant in urine culture?

Sensitive means the microorganism present in the urine can't grow in the presence of a particular antibiotic or the antibiotic is effective against that microorganism. Resistant means the microorganism can grow even if the antibiotic is present. This is a sign of an ineffective antibiotic. Your doctor may not prescribe this antibiotic in such a case.

Q. How is the Urine C/S (Urine Culture and Sensitivity) test different from the urine routine/microscopy (R/M) test?

The Urine C/S (Urine Culture and Sensitivity) test specifically detects the presence of bacteria or fungi in the urine and identifies the most effective antibiotics for UTI treatment. In contrast, a urine routine/microscopy (R/M) test is a general screening test that checks for physical, chemical, and microscopic elements in urine.

Q. Can the Urine C/S (Urine Culture and Sensitivity) test detect sexually transmitted diseases?

No, this test cannot detect sexually transmitted diseases.

Q. What can affect the results of the Urine C&S test?

The results of a Urine C&S test can get affected if the patient has recently taken antibiotics before the sample collection. It may also be affected if the urine sample is not collected properly. These may result in False negative or false positive results on urine culture.

Q. Can I have a negative urine culture test even with symptoms of UTI?

A negative/normal urine culture test result means that the urine sample showed no significant growth of bacteria or yeast on culture. However, if symptoms persist, a urine culture may be repeated on another sample to look for the presence of bacteria at lower colony counts. Inform the laboratory if you are already started on antibiotic therapy by your doctor, as this will affect test results. Please share your prescription if available with you.

Q. What does “mixed growth” mean in culture?

Sometimes, your culture shows several types of bacteria (mixed growth); this growth is likely due to contamination from outside your urinary tract or the surrounding skin or vaginal contamination in women in voided urine samples. If symptoms persist, your doctor may order a repeat culture on a more carefully collected “mid-stream clean catch” urine sample.

Q. Can I have WBC/ pus cells in urine routine examination, but no organism on culture?

Sterile pyuria can lead to high levels of white blood cells in urine without the presence of bacteria. It has been linked to various causes, such as recently treated UTI (usually within 2 weeks), sexually transmitted infections (STIs), cystitis, atypical infections like tuberculosis (TB), autoimmune diseases like systemic lupus erythematosus (SLE), drug intake and malignancy.

Q. What is the composition of urine?

Urine is composed of water (95%), with the rest being urea, creatinine, salts, and waste products from metabolism.

Q. What is the normal pH of urine?

The pH of urine typically ranges between 4.5 and 8.0. Diet, hydration, and infection can affect this value.

Q. What do pus cells in urine mean?

Pus cells in urine (also called white blood cells in urine) indicate infection or inflammation in the urinary tract.

Q. What do yeast cells in urine indicate?

Yeast cells in urine may indicate a fungal infection, which cannot be treated with antibiotics.

Q. What do crystals in urine indicate?

Crystals in urine can occur naturally but may also indicate kidney stones or metabolic issues if seen in excess.

Q. What does the urine culture test cost?

The cost of a urine culture test can vary based on the diagnostic lab, location, and whether it's part of a broader testing package.
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Urine C/S (Urine Culture and Sensitivity) test price for other cities


Price inBangaloreRs. 639
Price inNew DelhiRs. 769
Price inKolkataRs. 509
Price inHyderabadRs. 708
Price inMumbaiRs. 769
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References

  1. Urine Culture [Internet]. Rochester, NY: University of Rochester Medical Center; [Accessed 23 Feb. 2023]. Available from: https://www.urmc.rochester.edu/encyclopedia/content.aspx?contenttypeid=167&contentid=urine_cultureExternal Link
  2. Szlachta-McGinn A, Douglass K, Chung U, et al. Molecular Diagnostic Methods Versus Conventional Urine Culture for Diagnosis and Treatment of Urinary Tract Infection: A Systematic Review and Meta-analysis. European Urology Open Science. Oct. 2022;22:113-124. [Accessed 23 Feb. 2023]. Available from: https://www.sciencedirect.com/science/article/pii/S2666168322008801External Link
  3. Becker GJ, Garigali G, Fogazzi GB. Advances in Urine Microscopy. Am J Kidney Dis. 2016;67(6):954-964. [Accessed 23 Feb. 2023]. Available from: https://www.sciencedirect.com/science/article/abs/pii/S0272638615014262External Link
  4. Lamchiagdhase P, Preechaborisutkul K, Lomsomboon P, Srisuchart P, Tantiniti P, Khan-u-ra N, Preechaborisutkul B. Urine sediment examination: A comparison between the manual method and the iQ200 automated urine microscopy analyzer. Clin Chim Acta. 2005;358(1-2):167-174. [Accessed 23 Feb. 2023]. Available from: https://www.sciencedirect.com/science/article/abs/pii/S0009898105001518External Link
  5. Leber AL, editor. Clinical microbiology procedures handbook. 4th ed. Washington, D.C.: American Society for Microbiology; 2016. Available from: https://onlinelibrary.wiley.com/doi/pdf/10.1128/9781555818814.fmatterExternal Link
  6. Dutta C, Pasha K, Paul S, Abbas MS, Nassar ST, Tasha T, Desai A, Bajgain A, Ali A, Mohammed L. Urinary Tract Infection Induced Delirium in Elderly Patients: A Systematic Review. Cureus. 2022 Dec 8;14(12):e32321. Accessed 23 Apr. 2025]. Available from: https://pmc.ncbi.nlm.nih.gov/articles/PMC9827929/ External Link
  7. McCollum BJ, Garigan T, Earwood J. PURL: Can drinking more water prevent urinary tract infections? J Fam Pract. 2020 Apr;69(3):E19-E20. PMID: 32289134; PMCID: PMC7271893. Accessed 23 Apr. 2025]. Available from: https://pmc.ncbi.nlm.nih.gov/articles/PMC7271893/ External Link
  8. Cranberry Juice, Cranberry Tablets, or Liquid Therapies for Urinary Tract Infection: A Systematic Review and Network Meta-analysis [Internet]. ScienceDirect; [Accessed 23 Apr. 2025]. Available from: https://www.sciencedirect.com/science/article/pii/S2405456924001226 External Link
  9. 15 Tips To Keep Your Bladder Healthy [Internet]. NIA; 24 Jan. 2022 [Accessed 23 Apr. 2025]. Available from: https://www.nia.nih.gov/health/bladder-health-and-incontinence/15-tips-keep-your-bladder-healthy External Link
  10. Urinary Tract Infection Basics [Internet]. CDC; 22 Jan. 2024 Accessed 23 Apr. 2025]. Available from: https://www.cdc.gov/uti/about/index.html External Link
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