Test Detail
Understanding the Test
Test Measures
Interpreting Results
City Price Info
Home Collection
Other Tests
Content created by
Reviewed by
Dr. Swati Gupta (Anand)
MD Lab Medicine, MBBS
Want to know more?
Read our Editorial Policy

HLA B27 (Human Leukocyte Antigen B27) - PCR

Also known as Human leukocyte A antigen, Histocompatibility leukocyte A antigen
28593200 10% Off
You need to provide
This test is for
Male, Female
Test Preparation
  1. No special preparation is required.

Understanding HLA B27 (Human Leukocyte Antigen B27) - PCR

What is HLA B27 (Human Leukocyte Antigen B27) - PCR?

An HLA B27 (Human Leukocyte Antigen B27) - PCR test uses the Polymerase Chain Reaction (PCR) technique to detect the presence of the HLA B27 gene, a specific type of protein, on the surface of white blood cells. This gene is mainly associated with autoimmune and inflammatory conditions, such as ankylosing spondylitis.

Human Leukocyte Antigens (HLAs) genes play a crucial role in the immune system, and the HLA B27 variant is associated with certain autoimmune conditions, particularly seronegative spondyloarthropathies, a group of inflammatory rheumatic conditions, including ankylosing spondylitis, reactive arthritis, psoriatic arthritis, and juvenile idiopathic arthritis. 

A HLA B27 (Human Leukocyte Antigen B27) - PCR test assists in the detection, disease risk assessment, and monitoring of these conditions. The Polymerase Chain Reaction (PCR) technique is more sensitive and specific than other techniques like flow cytometry, allowing for accurately identifying the HLA-B27 gene.

Your doctor may suggest a HLA B27 (Human Leukocyte Antigen B27) - PCR test if you exhibit signs and symptoms such as chronic back pain, joint pain, stiffness, or inflammation in the eyes, urethra, and joints or have a family history of autoimmune conditions. The HLA B27 test is also used to match donated tissue to the tissue of the organ transplant recipient.

Usually, no special preparation is needed for this test. You may eat and drink as per your daily routine. Talk to your doctor about your specific test results. Narrate your complete medical history to help them correlate your clinical and laboratory findings. The test results will help them determine your medical condition and formulate an overall treatment plan. However, the HLA B27 test results are not interpreted as proof of whether you have an autoimmune condition or not, whether they are positive or negative.

What is HLA B27 (Human Leukocyte Antigen B27) - PCR used for?

An HLA B27 (Human Leukocyte Antigen B27) - PCR test is done:

  • If an individual exhibits symptoms of arthritis, such as chronic joint pain, stiffness, inflammation in the lower back, hips, neck, and chest, etc., or uveitis (painful inflammation of the eyes).
  • In individuals with a family history of autoimmune diseases.
  • Before an organ transplant procedure.
  • To monitor disease and treatment progress in individuals with pre-existing autoimmune disorders.

What does HLA B27 (Human Leukocyte Antigen B27) - PCR measure?

An HLA B27 (Human Leukocyte Antigen B27) - PCR test is used to determine the presence or absence of HLA B27 antigen to rule out the possibility of an autoimmune disorder. The presence of HLA-B27 protein on the surface of white blood cells is often associated with autoimmune disorders. A positive result is associated with an increased risk of developing certain autoimmune conditions, particularly ankylosing spondylitis, reactive arthritis, and psoriatic arthritis. A negative result suggests the absence of the HLA-B27 gene variant. However, it does not rule out the possibility of developing an autoimmune disorder, as not all affected individuals carry the HLA-B27 gene.

Interpreting HLA B27 (Human Leukocyte Antigen B27) - PCR results






No expression of HLA B-27


Grey zone (within 10 channels of cutoff)


Expression of HLA B-27 detected

Anti-HLA-B27 antibody cross-reacts with several other antigens of the HLA-B group, specially HLA-B7, leading to false-positive results*

Answers to Patient Concerns & Frequently Asked Questions (FAQs) about HLA B27 (Human Leukocyte Antigen B27) - PCR

Frequently Asked Questions about HLA B27 (Human Leukocyte Antigen B27) - PCR

Q. Why is the {test-name} test done?

The HLA B27 (Human Leukocyte Antigen B27) - PCR test is done if you have symptoms like pain and inflammation associated with spine, neck, and chest stiffness. This test helps determine whether you have human leukocyte antigen B27 (HLA B27) on the surface of your cells, and this, in turn, helps assess the possibility that you may be suffering from an autoimmune disorder.

Q. Is there any risk associated with the HLA B27 test?

The HLA B27 test is a safe procedure. However, there are some common risks involved when having blood drawn from the arm, such as mild pain, bruising, or bleeding at the injection site. Most of these symptoms go away in a day or two. Serious complications are rare.

Q. What diseases are associated with positive HLA B27 test results?

There are various diseases that are associated with positive HLA B27 test results. These are ankylosing spondylitis, psoriatic arthritis, reactive arthritis, uveitis, sacroiliitis, and arthritis related to Crohn's disease or ulcerative colitis.

Q. What other tests might be performed alongside the HLA B27 (Human Leukocyte Antigen B27) - PCR?

Depending on your symptoms and medical history, other tests may be conducted, such as rheumatoid factor (RF), anti-cyclic citrullinated peptide (anti-CCP), erythrocyte sedimentation rate (ESR), or C-reactive protein (CRP) test.

Q. What is reactive arthritis?

Reactive arthritis is a painful form of inflammatory arthritis. This includes joint disease caused due to inflammation. It develops due to inflammation caused in response to infection which is caused by certain bacteria that are present in the genitals (Chlamydia trachomatis) or the bowel (Campylobacter, Shigella, Salmonella, and Yersinia).

Q. What is ankylosing spondylitis?

Ankylosing spondylitis is a form of arthritis that primarily affects the spine but can also affect other joints. It comprises spinal joint inflammation, which can further cause severe, chronic pain and discomfort. If the inflammation is left undiagnosed, it can lead to new bone formation in the spine, known as ankylosis. In ankylosis, sections of the spine fuse in a fixed and immobile position. In this, one can develop inflammation, pain, and stiffness in other body parts, such as hips, ribs, shoulders, heels, and small joints of hands and feet. The inflammation can also involve eyes, known as uveitis or iritis. However, in rare cases, the heart and lungs can be affected.

Q. Is it possible to have a positive HLA B27 and not have any disease?

It is possible to have a positive HLA B27 without any specific disease. Some individuals have the HLA B27 protein but never develop an associated disease.

Q. Can HLA B27 be cured?

No, the presence of the HLA B27 protein on the surface of your white blood cells cannot be cured by reversing the presence of the antigen. However, this condition can be managed well to live a long and healthy life.

Q. What is the best way to lower down the elevated levels of human leukocyte antigen?

Always consult your doctor when your test results show abnormal test results. However, if you have been diagnosed with an autoimmune disease, your doctor may recommend that you take medication to lower your HLA b27 test level. Adopting some proactive measures such as eating a healthy diet, regular exercise, and reducing stress may also help to lower your HLA b27 test levels
+ more

HLA B27 (Human Leukocyte Antigen B27) - PCR test price for other cities

Price inBangaloreRs. 2859
Price inMumbaiRs. 2859
Price inKolkataRs. 2859
Price inGurgaonRs. 2859
Price inNoidaRs. 3040
+ more

Book HLA B27 (Human Leukocyte Antigen B27) - PCR at-home

Easy online booking
Search for tests and packages, book a time and select address for seamless at-home lab tests.
Live tracking of phlebotomist
Stay informed with live tracking of our phlebotomist's location for seamless sample collection.
Safe collection
Our phlebotomists follow strict safety protocols to collect samples at home on time.
Sample received at lab
Your sample is bought to our laboratory for testing by our qualified experts.
Quick reports, free follow up
Reports are sent to your email. A free doctor follow up is provided to understand the report better.


  1. Parameswaran P, Lucke M. HLA-B27 Syndromes. [Updated 2023 Jul 4]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2023 Jan-. Available from: https://www.ncbi.nlm.nih.gov/books/NBK551523/External Link
  2. Zochling J, Bohl-Bühler MH, Baraliakos X, Feldtkeller E, Braun J. Infection and work stress are potential triggers of ankylosing spondylitis. Clin Rheumatol. 2006 Sep;25(5):660-6.[Accessed 06 Nov. 2023]. Available from: https://pubmed.ncbi.nlm.nih.gov/16633712/ External Link
  3. HLA- B27 Antigen [Internet]. Rochester, NY: University of Rochester Medical Center; [Accessed 06 Nov. 2023]. Available from: https://www.urmc.rochester.edu/encyclopedia/content.aspx?ContentTypeID=167&ContentID=hla_b27_antigen External Link
  4. N P, Shanmugam SG, Devi SS, Chinambedu Dandapani MP, S R, D'Cruze L. Detection of Human Leukocyte Antigen B27 by Flowcytometry in Patients With Suspected Ankylosing Spondylitis in a Tertiary Care Centre. Cureus. 2021 Feb 25;13(2):e13560. [Accessed 06 Nov. 2023]. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8004549/#:~:text=HLA%2DB27%20testing%20is%20routinely%20used%20to%20diagnose%20AS%20since,%2Dpositive%20cases%20%5B12%5D. External Link
  5. McMichael A, Bowness P. HLA-B27: natural function and pathogenic role in spondyloarthritis. Arthritis Res. 2002;4 Suppl 3(Suppl 3):S153-8. [Accessed 06 Nov. 2023]. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3240147/ External Link
  6. Ankylosing Spondylitis. [Internet]. NIH; May. 2023. [Accessed 06 Nov. 2023]. Available from: https://www.niams.nih.gov/health-topics/ankylosing-spondylitis#:~:text=Ankylosing%20spondylitis%20is%20a%20type,help%20us%20move%20and%20bend. External Link


Orders Delivered
Get the link to download App

All products displayed on Tata 1mg are procured from verified and licensed pharmacies. All labs listed on the platform are accredited


Tata 1mg uses Secure Sockets Layer (SSL) 128-bit encryption and is Payment Card Industry Data Security Standard (PCI DSS) compliant


Find affordable medicine substitutes, save up to 50% on health products, up to 80% off on lab tests and free doctor consultations.

Know more about Tata 1mgdownArrow

Access medical and health information

Tata 1mg provides you with medical information which is curated, written and verified by experts, accurate and trustworthy. Our experts create high-quality content about medicines, diseases, lab investigations, Over-The-Counter (OTC) health products, Ayurvedic herbs/ingredients, and alternative remedies.

Order medicines online

Get free medicine home delivery in over 1800 cities across India. You can also order Ayurvedic, Homeopathic and other Over-The-Counter (OTC) health products. Your safety is our top priority. All products displayed on Tata 1mg are procured from verified and licensed pharmacies.

Book lab tests

Book any lab tests and preventive health packages from certified labs and get tested from the comfort of your home. Enjoy free home sample collection, view reports online and consult a doctor online for free.

Consult a doctor online

Got a health query? Consult doctors online from the comfort of your home for free. Chat privately with our registered medical specialists to connect directly with verified doctors. Your privacy is guaranteed.