ANA IFA Reflex to ANA / ENA Blot 18 Antigens (Qualitative) Profile Blot / LIA

An ANA IFA Reflex to ANA / ENA Blot 18 Antigens (Qualitative) Profile Blot / LIA helps to detect the antibodies that specifically target components within the cell nucleus. The nucleus of the cell houses the genetic material and is central to cellular function. Antibodies produced against the nucleus are especially important for understanding and addressing cellular activities. This test is instrumental in identifying various autoimmune disorders, including systemic lupus erythematosus (SLE), Sjogren's syndrome, Rheumatoid arthritis (RA), Scleroderma, and more. 

An ANA IFA Reflex to ANA / ENA Blot 18 Antigens (Qualitative) Profile Blot / LIA involves a two step process, starting with the antinuclear antibodies (ANA) by Immunofluorescence Assay (IFA) method and progressing to subsequent evaluation using an another method called line immunoassay (LIA) to qualitatively analyze 18 specific antigens associated with autoimmune disorders.

The initial ANA IFA test provides a broad overview, detecting the presence of antinuclear antibodies in the blood sample. If this test is positive, then further testing following the reflex mechanism directs the analysis to a detailed examination of 18 antigens, collectively called Extractable Nuclear Antigens (ENA). These antigens are important biomarkers for autoimmune diseases such as Systemic Lupus Erythematosus (SLE) etc. However, if ANA IFA test is negative then further testing of ANA/ ENA 18 Antigens by LIA method will not be done and only ANA IFA report shall be released. 

By identifying specific antibodies associated with various autoimmune disorders, an ANA IFA Reflex to ANA / ENA Blot 18 Antigens (Qualitative) Profile Blot / LIA helps in categorizing and understanding the nature of the autoimmune response in an individual’s body. 

An ANA IFA Reflex to ANA / ENA Blot 18 Antigens (Qualitative) Profile Blot / LIA  is typically suggested when a doctor suspects an autoimmune disorder due to symptoms such as joint pain, fatigue, skin rashes, or inflammation. Also, it may be advised if there are risk factors such as aging, obesity and a family history of autoimmune diseases.

Generally, no special preparations are needed for this test. However, it's advised to inform your doctor about any medications or supplements that you are taking, as certain medications may affect the test results. Additionally, telling a detailed medical history and discussing symptoms can help the doctor correlate the clinical and laboratory findings. 

The test results may vary depending on various factors like age, medical history, and certain risk factors among others. Abnormal test results require an expert interpretation; therefore, never try to self-medicate at home based solely on these results, and always consult a doctor for a proper understanding of the test results. The insights from this test helps the doctor tailor appropriate treatment plan.