
Hemoglobin HPLC/Electrophoresis with CBC near me in Dombivli










The Hemoglobin HPLC/Electrophoresis with CBC test is a specialized blood test used to identify and quantify different types of hemoglobin present in blood using HPLC along with complete blood count (CBC). The test helps identify abnormal hemoglobin variants and assess red blood cell characteristics, supporting the screening and diagnosis of inherited blood disorders, such as thalassemia and sickle cell disease. It is available at an affordable price in Dombivli with Tata 1mg Labs.








What does Hemoglobin HPLC/Electrophoresis with CBC measure?
Contains 22 testsThe Hemoglobin HPLC/Electrophoresis with CBC test identifies and measures different types of hemoglobin in the blood and assesses blood cell parameters through a CBC to help diagnose and keep track of certain blood disorders. These types of hemoglobin include: adult type (HbA2), fetal type (HbF), hemoglobin S (HbS), hemoglobin C (HbC), and hemoglobin E (HbE), among others.
The CBC measures parameters such as:
- Hemoglobin (Hb): Measures the amount of hemoglobin in the blood.
- Hematocrit (HCT): Measures the proportion of blood made up of red blood cells.
- Red blood cell (RBC) count: Measures the number of red blood cells in the blood.
- Red blood cell indices: Include MCV, MCH, MCHC, and RDW, which provide information about the size, hemoglobin content, and variation in the size of red blood cells.
- White blood cell (WBC) count and platelet count: Provide information about other blood cell types.
Normal hemoglobin types:
- Hemoglobin A (HbA): The main type of hemoglobin in healthy adults.
- Hemoglobin F (HbF): Found in unborn babies and newborns. It is slowly replaced by HbA after birth.
If the levels of HbA or HbF are not normal, it may indicate certain types of anemia. The interpretation depends on the overall hemoglobin pattern and CBC findings.
Abnormal hemoglobin types:
- Hemoglobin S (HbS): Found in people with sickle cell anemia. It causes red blood cells to become stiff and sickle-shaped, leading to pain and other health problems.
- Hemoglobin C (HbC): Linked to a condition called hemolytic anemia, where red blood cells break down faster than normal.
- Hemoglobin E (HbE): Common in people from Southeast Asia. It may cause mild anemia or sometimes no symptoms at all in the heterozygous state.
- Hemoglobin D (HbD): Causes mild hemolytic anemia and mild to moderate spleen enlargement. The Hb D Punjab variant is most commonly seen in people from Gujarat and among Sikhs of Punjab.
The HPLC or electrophoresis pattern, together with CBC findings and clinical history, helps the doctor assess whether further testing is required.
The CBC (Complete Blood Count) test measures the number of red blood cells (RBCs), white blood cells (WBCs), and platelets. Each of these blood cells performs essential functions–RBCs carry oxygen from your lungs to the various body parts, WBCs help fight infections and other diseases, and platelets help your blood to clot. Therefore, determining their levels can provide significant health information. A CBC test also determines the hemoglobin level, a protein in RBC that carries oxygen from the lungs to the rest of your body. Evaluating all these components together can provide important information about your overall health.
Know more about CBC (Complete Blood Count)
Differential Leukocyte Count
- Differential Neutrophil Count
- Differential Lymphocyte Count
- Differential Monocyte Count
- Differential Eosinophil Count
- Differential Basophil Count
There are five types of WBCs: neutrophils, lymphocytes, monocytes, eosinophils, and basophils. A Differential Leukocyte Count test measures the percentage of each type of WBC in the blood. Leukocytes or WBCs are produced in the bone marrow and defend the body against infections and diseases. Each type of WBC plays a unique role to protect against infections and is present in different numbers.
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Red Blood Cell Count
The Red Blood Cell Count test measures the total number of red blood cells in your blood. RBCs are the most abundant cells in the blood with an average lifespan of 120 days. These cells are produced in the bone marrow and destroyed in the spleen or liver. Their primary function is to help carry oxygen from the lungs to different body parts. The normal range of RBC count can vary depending on age, gender, and the equipment and methods used for testing.
Hb (Hemoglobin)
The Hb (Hemoglobin) test measures the concentration of hemoglobin in your blood. Hemoglobin binds oxygen molecules and carries them to body tissues while removing carbon dioxide. Low hemoglobin suggests reduced oxygen-carrying capacity. It can be due to anemia, bleeding, nutritional deficiencies (iron, vitamin B12, folate), or chronic disease. High hemoglobin may occur in dehydration, living at high altitudes, smoking, or diseases like polycythemia vera.
Platelet Count
The Platelet Count test measures the average number of platelets in the blood. Platelets are disk-shaped tiny cells originating from large cells known as megakaryocytes, which are found in the bone marrow. After the platelets are formed, they are released into the blood circulation. Their average life span is 7-10 days.
Platelets help stop the bleeding, whenever there is an injury or trauma to a tissue or blood vessel, by adhering and accumulating at the injury site and releasing chemical compounds that stimulate the gathering of more platelets. A loose platelet plug is formed at the site of injury and this process is known as primary hemostasis. These activated platelets support the coagulation pathway that involves a series of steps, including the sequential activation of clotting factors; this process is known as secondary hemostasis. After this step, there is a formation of fibrin strands that form a mesh incorporated into and around the platelet plug. This mesh strengthens and stabilizes the blood clot so that it remains in place until the injury heals. After healing, other factors come into play and break the clot down so that it gets removed. In case the platelets are not sufficient in number or not functioning properly, a stable clot might not form. These unstable clots can result in an increased risk of excessive bleeding.
Total Leukocyte Count
The Total Leukocyte Count test measures the numbers of all types of leukocytes, namely neutrophil, lymphocyte, monocyte, eosinophil, and basophil, in your blood. Leukocytes or WBCs are an essential part of our immune system. These cells are produced in the bone marrow and defend the body against infections and diseases. Each type of WBC plays a unique role to protect against infections and is present in different numbers.
Hematocrit
The Hematocrit test measures the proportion of red blood cells (RBCs) in your blood as a percentage of the total blood volume. It is a crucial part of a complete blood count (CBC) and helps in assessing your blood health. RBCs are responsible for carrying oxygen from the lungs to different parts of the body. The hematocrit test provides valuable information about your blood's oxygen-carrying capacity.
Higher-than-normal amounts of RBCs produced by the bone marrow can cause the hematocrit to increase, leading to increased blood density and slow blood flow. On the other hand, lower-than-normal hematocrit can be caused by low production of RBCs, reduced lifespan of RBCs in circulation, or excessive bleeding, leading to a reduced amount of oxygen being transported by RBCs. Monitoring your hematocrit levels is essential for diagnosing and managing various blood-related disorders.
Mean Corpuscular Volume
The Mean Corpuscular Volume test measures the average size of your red blood cells, which carry oxygen through your body. This test tells whether your RBCs are of average size and volume or whether they are bigger or smaller.
Mean Corpuscular Hemoglobin
An MCH test measures the average amount of hemoglobin in a single red blood cell (RBC). Hemoglobin is an iron-containing protein in RBCs, and its major function is to transport oxygen from the lungs to all body parts. This test provides information about how much oxygen is being delivered to the body by a certain number of RBCs.
Mean Corpuscular Hemoglobin Concentration
An MCHC test measures the average amount of hemoglobin in a given volume of RBCs. MCHC is calculated by dividing the amount of hemoglobin by hematocrit (volume of blood made up of RBCs) and then multiplying it by 100.
Absolute Leucocyte Count
- Absolute Lymphocyte Count (ALC)
- Absolute Neutrophil Count
- Absolute Basophil Count
- Absolute Monocyte Count
- Absolute Eosinophil Count
The Absolute Leucocyte Count test measures the total number of white blood cells (leucocytes) in the given volume of blood. It examines different types of white blood cells such as neutrophils, lymphocytes, monocytes, basophils and eosinophils. These cells tell about the status of the immune system and its ability to fight off infections and other conditions like inflammation, allergies, bone marrow disorders etc.
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Mean Platelet Volume
An MPV test measures the average size of the platelets in your blood. Platelets are disk-shaped tiny cells originating from large cells known as megakaryocytes, which are found in the bone marrow. After the platelets are formed, they are released into the blood circulation. Their average life span is 7-10 days.
Platelets help stop bleeding whenever there is an injury or trauma to a tissue or blood vessel by adhering and accumulating at the injury site, and by releasing chemical compounds that stimulate the gathering of more platelets. After these steps, a loose platelet plug is formed at the site of injury, and this process is known as primary hemostasis. These activated platelets support the coagulation pathway that involves a series of steps including the sequential activation of clotting factors; this process is known as secondary hemostasis. After this, there is a formation of fibrin strands that form a mesh incorporated into and around the platelet plug. This mesh strengthens and stabilizes the blood clot so that it remains in place until the injury heals. After healing, other factors come into play and break the clot down so that it gets removed. In case the platelets are not sufficient in number or are not functioning properly, a stable clot might not form. These unstable clots can result in an increased risk of excessive bleeding.
PDW
The PDW test reflects variability in platelet size, and is considered a marker of platelet function and activation (clot formation in case of an injury). This marker can give you additional information about your platelets and the cause of a high or low platelet count. Larger platelets are usually younger platelets that have been recently released from the bone marrow, while smaller platelets may be older and have been in circulation for a few days. Higher PDW values reflect a larger range of platelet size, which may result from increased activation, destruction and consumption of platelets.
RDW CV
The RDW CV test which is part of red cell indices, helps identify characteristics of red blood cells. RDW (red cell distribution width) measures the variations in the sizes of red blood cells, indicating how much they differ from each other in a blood sample. RDW is expressed as RDW-CV, a coefficient of variation. A higher RDW may suggest more variation in red cell sizes, while a lower RDW indicates more uniform red cell sizes.





FAQs related to Hemoglobin HPLC/Electrophoresis with CBC in Dombivli
- Wajcman H, Moradkhani K. Abnormal haemoglobins: detection & characterization. Indian J Med Res. 2011 Oct;134(4):538-46. PMID: 22089618; PMCID: PMC3237254. [Accessed 30 Oct. 2023]. Available from:
- Kohne E. Hemoglobinopathies: clinical manifestations, diagnosis, and treatment. Dtsch Arztebl Int. 2011 Aug;108(31-32):532-40. [Accessed 30 Oct. 2023]. Available from:
- Forget BG, Bunn HF. Classification of the disorders of hemoglobin. Cold Spring Harb Perspect Med. 2013 Feb 1;3(2):a011684. [Accessed 30 Oct. 2023]. Available from:
- Khera R, Singh T, Khuana N, Gupta N, Dubey AP. HPLC in characterization of hemoglobin profile in thalassemia syndromes and hemoglobinopathies: a clinicohematological correlation. Indian J Hematol Blood Transfus. 2015 Mar;31(1):110-5. [Accessed 30 Oct. 2023]. Available from:
- What is Thalassemia? [Internet]. CDC; 24 April 2023; [Accessed 30 Oct. 2023]. Available from:
- What is Sickle Cell Disease? [Internet]. CDC; 06 July 6 2023; [Accessed 30 Oct. 2023]. Available from:
- Billett HH. Hemoglobin and Hematocrit. In: Walker HK, Hall WD, Hurst JW, editors. Clinical Methods: The History, Physical, and Laboratory Examinations. 3rd edition. Boston: Butterworths; 1990. Chapter 151. Available from:
- Ahmed MH, Ghatge MS, Safo MK. Hemoglobin: Structure, Function and Allostery. Subcell Biochem. 2020;94:345-382. [Accessed 30 Oct. 2023]. Available from:
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