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Vitamin Profile

Also known as Multivitamins
Vitamin Profile Test Includes 3 testsView All
12702228 42% Off
You need to provide
This test is for
Male, Female
Test Preparation
  1. Overnight fasting is preferred.

Understanding Vitamin Profile

What is Vitamin Profile?

A Vitamin Profile is a group of tests that assesses the levels of Vitamin B12 (cobalamin), Vitamin D, and Vitamin B9 (folate) in the body. These tests help evaluate the nutritional status, detect vitamin deficiencies, and monitor the effectiveness of supplementation or dietary changes.

Vitamins B9 (folate), B12 (cobalamin), and D are crucial in various physiological functions, including energy metabolism, bone health, and DNA synthesis. Hence, determining their levels can provide significant health information. Vitamin B12 (cobalamin) and B9 (folate) are essential for  DNA synthesis and the formation of red blood cells. Furthermore, Vitamin B12 is also vital for neurological function. Vitamin D is crucial for healthy bones and teeth, immune function, and overall well-being.

Your doctor may advise a Vitamin Profile if you experience symptoms like fatigue, dizziness, joint or muscle pain, sudden weight changes, hair loss, frequent infections, and lower back pain. Additionally, this test may also help monitor the nutritional status of individuals with medical conditions that make them more prone to nutritional deficiencies, such as bone disorders, diabetes, obesity, and high cholesterol etc.

Abnormal Vitamin Profile results indicate deficiencies that may require supplementation or dietary adjustments. Vitamin B12 (cobalamin) deficiency can lead to anemia and neurological problems, among other health conditions. It may result from malabsorption, inadequate dietary intake (especially in vegetarians and vegans), and some medical conditions that affect the stomach or intestines. The risk factors for Vitamin D deficiency include limited sun exposure, dark skin, obesity, malabsorption disorders, and inadequate dietary intake. Low Vitamin D levels can lead to conditions like rickets in children, osteomalacia in adults, and an increased risk of fractures. Vitamin B9 or folate deficiency can occur due to inadequate dietary intake, malabsorption, alcoholism, and certain medications and may lead to megaloblastic anemia or neural tube defects in newborns.

Overnight fasting is preferred for this test. However, talk to your doctor about other precautions before taking the test. Test result ranges are approximate and may differ slightly between labs. Talk to your doctor about your specific test results. The results will help them determine your medical condition and recommend dietary modifications and supplementation to optimize vitamin levels and support your overall well-being.

What is Vitamin Profile used for?

A Vitamin Profile is done:

  • To check the levels of vitamins B9, B12, and D.
  • To monitor vitamin levels in individuals with chronic conditions like high blood pressure, diabetes, high cholesterol, etc.
  • In Individuals with symptoms such as fatigue, weakness, bone pain, muscle weakness, numbness, burning, or tingling sensation in hands, feet, or arms.
  • To assess nutritional status during pregnancy, adequate levels are essential to prevent complications such as neural tube defects in the developing fetus.

What does Vitamin Profile measure?

Contains 3 tests

A Vitamin Profile covers testing of three vitamins, including vitamins B9, B12, and D. These vitamins are required by your body for the proper functioning of the nervous system, cell growth, DNA formation, building immunity, bone health, and maintaining your overall health. Deranged levels of these vitamins can give rise to lethargy, irritation, muscle spasms, fatigue, anemia, psychological disorders, or toxicity if not given timely attention.

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Vitamin B 9

A Vitamin B 9 test analyzes the levels of vitamin B9 in the blood. Vitamin B9 is a part of the B complex of vitamins and is an essential nutrient, meaning the body cannot produce it and has to be taken into the diet. Folate is the naturally occurring form of vitamin B9, whereas folic acid refers to a supplement that is added to food and drinks. The deficiency of vitamin B9 (folic acid) can also lead to macrocytic anemia in which the size of the RBCs becomes larger than normal.

Know more about Vitamin B 9

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Vitamin B12

A Vitamin B12 test measures your vitamin B12 levels. Vitamin B12 is essential for various health aspects, such as maintaining a healthy nervous system, making red blood cells, and creating the genetic material of our cells. Low vitamin B12 levels are more likely to occur in older adults, children, vegans, vegetarians, people with diabetes, individuals who underwent gastric bypass surgery, women who are breastfeeding, and in conditions that impact absorption of this vitamin, like Crohn’s disease. Higher vitamin B12 levels are uncommon as excessive vitamin B12 is usually removed through the urine. However, some conditions, such as liver diseases and myeloproliferative disorders, can cause an increase in vitamin B12 levels, thereby affecting blood cell production.

Know more about Vitamin B12

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Vitamin D (25-Hydroxy)

A Vitamin D (25-Hydroxy) test measures the levels of vitamin D in the body. It is an essential nutrient that can be synthesized in the body upon healthy exposure to sunlight or absorbed from dietary sources. It majorly exists in two forms: Vitamin D2 (ergocalciferol) and Vitamin D3 (cholecalciferol). Vitamin D2 is present in plants, such as yeast or mushrooms, and is available as a supplement in fortified foods, and vitamin D3 is found in foods like cheese, green vegetables, mushrooms, egg yolks, and fatty fish.

Both forms of vitamin D (D2 and D3) need to undergo some chemical changes before being available for use in the body. These chemical changes take place in the liver or kidneys. The liver converts vitamin D to 25-hydroxyvitamin D (25-OH vitamin D). A Vitamin D (25-Hydroxy) test measures the level of this 25-OH vitamin D as it is the primary form of vitamin D that circulates in the blood.

Know more about Vitamin D (25-Hydroxy)

Interpreting Vitamin Profile results


The normal range of Vitamin B12 is 211 - 911 pg/mL, irrespective of sex and age.

High levels of Vitamin B12 may be seen in:

      • Kidney failure

      • Diabetes

      • Liver disease

Low levels of Vitamin B12 may be seen in:

      • Anemia

      • Hyperthyroidism (high levels of thyroid)

      • Worm infection


The normal range of vitamin D  (25-OH) is measured as nanograms per milliliter (ng/mL) or nmol/L and can vary from lab to lab.


Reference range (ng/mL)









      • There can be seasonal variations in vitamin D (25-OH) levels. The values can be 40-50% lower in winter than in summer. It is also influenced by sunlight, latitude, skin pigmentation, sunscreen use, and hepatic function.

      • Vitamin D (25-OH) levels can vary with age.

      • Vitamin D (25-OH) levels increase during pregnancy.


The normal range of Vitamin B9 is measured as nanograms per milliliter (ng/mL) 

Result in ng/mL


0.35 - 0.37


3.38 - 5.38


> 5.38


    • Certain medicines like methotrexate and leucovorin can cause changes in vitamin B9 levels.

    • Methylmalonic acid in urine and serum homocysteine tests are suggested to differentiate between vitamin B9 and vitamin B12 deficiencies.

*Reference range may vary from lab to lab

Answers to Patient Concerns & Frequently Asked Questions (FAQs) about Vitamin Profile

Frequently Asked Questions about Vitamin Profile

Q. What does a Vitamin Profile measure?

A Vitamin Profile measures the quantity of vitamins B9, B12, and D in the body. These vitamins are required for the proper functioning of the nervous system, DNA formation, cell growth, RBC production, and absorption of calcium and phosphorous.

Q. What diseases are diagnosed with a Vitamin Profile?

A Vitamin Profile package that includes measurements of vitamins B9 (folate), B12 (cobalamin), and D (vitamin D) is typically used to diagnose and monitor various nutritional and health conditions related to these vitamins. Some of the diseases and conditions that can be diagnosed or assessed using a vitamin profile package include megaloblastic anemia, pernicious anemia, certain neurological disorders, rickets, hypocalcemia, and increased risk of chronic diseases like osteoporosis, autoimmune disorders, and some cancers.

Q. What are the deficiency symptoms of vitamins included in the Vitamin Profile package?

Some of the common symptoms of vitamin B9 deficiency include fatigue and weakness, pale skin, shortness of breath, reduced exercise tolerance, numbness and tingling in the extremities, difficulty with coordination and balance, changes in mood, cognitive impairment, and gastrointestinal issues like diarrhea, glossitis (Inflammation of the tongue, which can cause a sore or swollen tongue), or stomatitis. Common symptoms of vitamin B12 deficiency include fatigue and weakness; pale or jaundiced (yellowish) skin; shortness of breath; dizziness or lightheadedness; rapid heart rate; numbness and tingling, especially in the hands and feet; balance problems and difficulty walking; muscle weakness; coordination difficulties; memory problems and cognitive changes; mood disturbances, including depression, irritability, and psychosis (in severe cases); and vision changes; glossitis and mouth ulcers; diarrhea or other gastrointestinal problems; and in rare cases, symptoms such as an enlarged heart, chest pain, or heart palpitations. The symptoms of vitamin D deficiency can vary in severity and may develop gradually over time. Some of the common symptoms of vitamin D deficiency are bone pain or tenderness; muscle aches and weakness; increased risk of fractures or falls; mood changes like depression, irritability, and anxiety; cognitive changes; chronic pain conditions, including fibromyalgia; increased risk of infections, particularly respiratory tract infections; hair loss or hair thinning; slow wound healing; and bone deformities in children.

Q. How is a Vitamin Profile performed?

A phlebotomist (a trained professional to perform blood draws) will clean your skin using an antiseptic alcohol cotton swab or wipe and take out a blood sample from your vein using a needle. The blood sample will be stored safely and transported to the laboratory for analysis.

Q. Is there any risk associated with a Vitamin Profile?

No, usually, there are no risks associated with this test. The blood sample is withdrawn with the help of a needle, and the process is fairly fast and relatively painless. Very rarely, you may experience excessive pain, bleeding, hematoma (blood collection under the skin), bruising, or infection at the site of the needle prick. Consult the doctor if you experience any of these.

Q. What is the benefit of getting a Vitamin Profile done?

This profile test helps identify the status of vitamins B9, B12, and D in your body and also allows your doctor to provide you with the required amount of vitamins as per your reports.

Q. Can I take a Vitamin Profile during pregnancy?

Yes, a Vitamin Profile during pregnancy may help detect any blood cell abnormalities in the mother and its associated risk in the baby.

Q. What can affect my Vitamin Profile results?

Certain medicines like methotrexate and leucovorin can affect the result of the vitamin B9 test. If you are taking vitamin supplements, talk to your doctor before this test.

Q. What are some of the food sources of vitamins B9, B12, and D?

Vitamins B9, B12, and D are essential nutrients, and including them in your diet is crucial for overall health. Here are some food sources for each of these vitamins: Vitamin B9 (Folate): Vegetables like spinach, kale, collard greens, asparagus, and broccoli; legumes like beans, lentils, and peas; fruits like oranges, grapefruits, and avocado; and fortified cereals, bread, and pasta. Vitamin B12 (Cobalamin): Animal-based foods, including meat (beef, pork, lamb), poultry (chicken, turkey), fish (salmon, trout, tuna), shellfish (clams, mussels, oysters); dairy products (milk, cheese, yogurt); eggs; and some plant-based foods, such as plant-based milk (e.g., almond milk, soy milk) and breakfast cereals. Vitamin D: Healthy exposure to sunlight; fatty fish like salmon, mackerel, sardines, and trout; cod liver oil; egg yolks; and fortified Foods like cow's milk, plant-based milk (e.g., almond milk, soy milk), breakfast cereals, orange juice, and yogurt. A balanced diet that includes a variety of foods from these sources can help ensure you get an adequate intake of vitamins B9, B12, and D. If you have specific dietary restrictions or concerns about your vitamin intake, consider consulting a registered dietitian or healthcare provider for personalized advice.
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Vitamin Profile test price for other cities

Price inBangaloreRs. 1270
Price inHyderabadRs. 1270
Price inMumbaiRs. 1270
Price inGurgaonRs. 1270
Price inPuneRs. 1270
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