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Vitamin D (25-Hydroxy) & Vitamin B12

Vitamin D (25-Hydroxy) & Vitamin B12

Also referred as
25-OH vitamin D
Calcidiol
Cyanocobalamin
+2
Methylcobalamin
Cobalamin
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2 tests
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The Vitamin D (25-Hydroxy) & Vitamin B12 test measures the level of vitamin D and vitamin B12 in your body. This test helps determine deficiencies related to vitamin D and B12. It also helps assess if you have too much of these vitamins in your body (most likely from excessive supplements). Vitamin D helps keep bones, teeth, and muscles healthy, whereas vitamin B12 maintains blood, brain, and nerve functions.

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Understanding Vitamin D (25-Hydroxy) & Vitamin B12

Vitamins, although required in small amounts, are significant in maintaining overall health and well-being.  Vitamin D (25-hydroxy) plays a critical role in various body functions, including:

  • Supports immune system functioning
  • Helps maintain healthy bones
  • Promotes cell growth

Vitamin D also acts as a hormone because it is synthesized in the skin (via sunlight) and then converted to its active form (known as calcitriol) in the kidneys. This active form helps regulate the levels of calcium and phosphorus in the body. Low vitamin D levels can result in an increased risk of fractures, muscle weakness and lethargy, dysfunction of the immune system, and an increased risk of diabetes.

Vitamin B12, also known as cobalamin, belongs to the B complex group of vitamins. It is vital for various body functions such as:

  • Aids in the production of red blood cells (RBCs)
  • Supports repair and regeneration of cells and tissues
  • Promotes optimal brain function and nerve health

Some common symptoms of vitamin B12 deficiency include fatigue, mouth ulcers, loss of appetite, burning sensation in feet, hands, arms, and legs, muscle weakness, etc.

The Vitamin D (25-Hydroxy) & Vitamin B12 checks for the levels of Vitamin D and Vitamin B12 in your body. The vitamin D test is important for assessing bone health and calcium metabolism. It helps detect deficiencies that can lead to rickets in children and osteomalacia or osteoporosis in adults. The vitamin B12 test helps determine the response to vitamin B12 therapy in conditions like anemia. Additionally, it is important to monitor parameters to maintain a healthy pregnancy. Low vitamin B12 levels have been associated with adverse pregnancy outcomes like anemia, low birth weight, and growth retardation of the baby.  The Vitamin D (25-Hydroxy) & Vitamin B12 test is also done to check for excessive vitamin D and B12, particularly due to overuse of supplementation.

Usually, no special preparation is required for this test. Eat and drink as per the daily routine. However, do let the doctor know about all the medications being taken, as some of them may affect the accuracy of the test results.

Test result ranges are approximate and may differ slightly between labs depending on the methodology and laboratory guidelines. Talk to the doctor about specific test results. They can help you understand what the results mean, suggest lifestyle changes like diet or exercise, give medicine if needed, and plan the right treatment for you.

Lifestyle Tips to Support Healthy Vitamin D and Vitamin B12 Levels:

🥤 Hydration: Drink enough water daily to support proper digestion and vitamin absorption.

🌞 Exposure to the sun: Get regular morning sunlight for natural vitamin D synthesis.  Avoid smoking and limit alcohol, as these can interfere with both vitamin D metabolism and vitamin B12 absorption.

🏃 Activity (Exercise): Regular physical activity, especially outdoors, supports vitamin D production and may enhance B12 metabolism.

🍔 Lifestyle (Balanced Diet): Include vitamin D-rich foods (like eggs, mushrooms, salmon, fortified milk) and vitamin B12-rich foods (like meat, fish, dairy, or fortified cereals for vegetarians/vegans).

👩‍⚕️ Talk to Your Doctor (Consultation): Avoid self-supplementation.  Consult your doctor for proper guidance on vitamin D or B12 levels and safe supplementation if needed.

🚭 Habits (Smoking and Alcohol): These habits can interfere with the absorption and activation of vitamins B12 and D.

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What does Vitamin D (25-Hydroxy) & Vitamin B12 measure?

Contains 2 tests

The Vitamin D (25-Hydroxy) & Vitamin B12 test is tailored to determine the levels of two important vitamins in your body: vitamin D (25-OH) and vitamin B12. Vitamin D is an essential nutrient that can be synthesized in the body upon healthy exposure to sunlight or absorbed from dietary sources. On the other hand, vitamin B12 is vital for maintaining a healthy nervous system, making red blood cells, and creating the genetic material of our cells.

This test is generally recommended as a part of routine health checkups, nutritional assessments, or to monitor vitamin levels in individuals with dietary restrictions or those who are on vitamin supplements.

The Vitamin B12 test measures your vitamin B12 levels. This vitamin is essential for various health aspects, such as maintaining a healthy nervous system, making red blood cells, and creating the genetic material of our cells. Low vitamin B12 levels are more likely to occur in older adults, children, vegans, vegetarians, people with diabetes, individuals who underwent gastric bypass surgery, women who are breastfeeding, and in conditions like Crohn’s disease that impact the absorption of this vitamin. High vitamin B12 levels are seen in conditions like liver diseases and myeloproliferative disorders. Also, high levels of vitamin B12 are seen in individuals on vitamin supplementation, but this usually does not require treatment as the excess vitamin is flushed out through urine.

Know more about Vitamin B12

The Vitamin D (25-Hydroxy) test measures the levels of vitamin D in the body. Vitamin D is an essential nutrient that can be synthesized in the body upon healthy exposure to sunlight or absorbed from dietary sources. It majorly exists in two forms: vitamin D2 (ergocalciferol) and vitamin D3 (cholecalciferol). Vitamin D2 is present in plants, such as yeast or mushrooms, and is available as a supplement in fortified foods, and vitamin D3 is found in foods like cheese, green vegetables, mushrooms, egg yolks, and fatty fish.

Vitamin D is available in two key forms: D2 (present in plants like mushrooms and in some fortified foods) and D3 (present in foods like cheese, green vegetables, egg yolks, mushrooms, and fatty fish).

Both forms of vitamin D need to be converted in the liver and kidneys before the body can use them effectively. This test shows the total level of vitamin D (D2 + D3) circulating in the body and guides your doctor in recommending the right treatment or lifestyle changes if needed.

Know more about Vitamin D (25-Hydroxy)

FAQs related to Vitamin D (25-Hydroxy) & Vitamin B12

The Vitamin D (25-Hydroxy) & Vitamin B12 test can be done at any time of the day as no fasting is required for getting tested.
No, the Vitamin D (25-Hydroxy) & Vitamin B12 test is generally a safe procedure. While rare, bruising, bleeding, and infection at the puncture site may occur. In very uncommon cases, there can be swelling of the vein after the blood withdrawal.
Bone pain, depression, hair loss, muscle weakness, appetite loss, pale skin, and sleep disturbances are some common signs and symptoms of vitamin D deficiency.
Insufficient sunlight exposure or a vitamin D-deficient diet are the primary cause of vitamin D deficiency.
Always refer to your doctor in case your test results indicate low levels of vitamin D. Although consuming oily fish such as salmon or sardines, red meat, egg yolks, and fortified cereals may help improve vitamin D levels. Additionally, sufficient exposure to the sun can assist you in naturally increasing your levels of vitamin D.
Muscle weakness, loss of appetite, vision problems, a painful tongue, mouth ulcers, a fast and irregular heartbeat, shortness of breath, tingling or numbness, and pins and needles or burning sensations in the feet, hands, arms, and legs are common signs and symptoms of vitamin B12 deficiency.
Deficiency of vitamin B12 could be due to poor dietary intake, pernicious anemia, and certain types of stomach-related surgeries, like weight loss surgery or intestinal surgery.  Some medicines and immune system problems like lupus or Graves' disease can also cause it. Talk to your doctor if you have any of these issues.
Low vitamin B12 levels can lead to symptoms like fatigue, weakness, pale skin, appetite loss, weight loss, infertility, numbness or tingling in hands and feet, balance issues, depression, confusion, memory problems, and mouth soreness. In infants, it may cause growth delays and anemia. Early treatment is crucial to prevent nerve damage.
Always consult your doctor if your vitamin B12 levels are lower than normal. Incorporating food like meat, fish, eggs, milk, cheese, soy products, and fortified cereals may also help boost your vitamin B12 levels.
Regular sun exposure is the most natural way to get enough vitamin D. Usually, you need midday sunlight for about 10 to 30 minutes several times weekly. However, darker-skinned people may need a little longer than this. Exposure time also depends on how sensitive your skin is to sunlight.
a) 0–12 months: 400 IU/day; b). Ages 1–70: 600 IU/day; c) Over 70: 800 IU/day. For adults with low levels (<30 ng/mL), 1,500–2,000 IU/day is advised to restore balance (as per Endocrine Society).
Yes, low Vitamin B12 levels can lead to mental health problems like depression, anxiety, confusion, and memory issues. Vitamin B12, along with other B vitamins, helps control brain chemicals that affect mood and behavior.
Pregnant women need enough nutrients to help the baby grow and develop well. Low Vitamin B12 levels can lead to problems like anemia, low birth weight, and slow growth in the baby. It may also cause serious issues like spina bifida (a problem with the baby’s spine) and poor brain development.

Vitamin D (25-Hydroxy) & Vitamin B12 test price for other cities

Price inBangaloreRs. 1149
Price inHyderabadRs. 649
Price inPuneRs. 1149
Price inMumbaiRs. 1149
Price inNew DelhiRs. 1149

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Contains 2 tests
Vitamin B12
Vitamin D (25-Hydroxy)