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Vitamin D (25-Hydroxy) & Vitamin B12

Vitamin D (25-Hydroxy) & Vitamin B12 near me in Jaipur

Also referred as
25-OH vitamin D
Calcidiol
Cyanocobalamin
+2
Methylcobalamin
Cobalamin
38,142+ booked recently
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Contains
2 tests
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The Vitamin D (25-Hydroxy) & Vitamin B12 package helps measure vitamin D and B12 levels in the blood at an affordable price in Jaipur with Tata 1mg labs. Vitamin D is a fat-soluble and essential nutrient that helps the body maintain strong bones & teeth and a healthy immune system. On the other hand, vitamin B12 is vital for healthy brain and nerve functions, energy production, and red blood cell formation. 

The Vitamin D (25-Hydroxy) & Vitamin B12 package is used to detect disorders associated with vitamin D and B12 deficiencies. However, your doctor can also suggest this test package to monitor the treatment efficacy of vitamin supplements and to manage your condition better.

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Understanding Vitamin D (25-Hydroxy) & Vitamin B12

Vitamins, although required in small amounts, are significant in maintaining overall health and well-being.  Vitamin D (25-hydroxy) plays a critical role in various body functions, including:

  • Supports immune system functioning
  • Helps maintain healthy bones
  • Promotes cell growth

Vitamin D also acts as a hormone because it is synthesized in the skin (via sunlight) and then converted to its active form (known as calcitriol) in the kidneys. This active form helps regulate the levels of calcium and phosphorus in the body. Low vitamin D levels can result in an increased risk of fractures, muscle weakness and lethargy, dysfunction of the immune system, and an increased risk of diabetes.

Vitamin B12, also known as cobalamin, belongs to the B complex group of vitamins. It is vital for various body functions such as:

  • Aids in the production of red blood cells (RBCs)
  • Supports repair and regeneration of cells and tissues
  • Promotes optimal brain function and nerve health

Some common symptoms of vitamin B12 deficiency include fatigue, mouth ulcers, loss of appetite, burning sensation in feet, hands, arms, and legs, muscle weakness, etc.

The Vitamin D (25-Hydroxy) & Vitamin B12 checks for the levels of Vitamin D and Vitamin B12 in your body. The vitamin D test is important for assessing bone health and calcium metabolism. It helps detect deficiencies that can lead to rickets in children and osteomalacia or osteoporosis in adults. The vitamin B12 test helps determine the response to vitamin B12 therapy in conditions like anemia. Additionally, it is important to monitor parameters to maintain a healthy pregnancy. Low vitamin B12 levels have been associated with adverse pregnancy outcomes like anemia, low birth weight, and growth retardation of the baby.  The Vitamin D (25-Hydroxy) & Vitamin B12 test is also done to check for excessive vitamin D and B12, particularly due to overuse of supplementation.

Usually, no special preparation is required for this test. Eat and drink as per the daily routine. However, do let the doctor know about all the medications being taken, as some of them may affect the accuracy of the test results.

Test result ranges are approximate and may differ slightly between labs depending on the methodology and laboratory guidelines. Talk to the doctor about specific test results. They can help you understand what the results mean, suggest lifestyle changes like diet or exercise, give medicine if needed, and plan the right treatment for you.

Lifestyle Tips to Support Healthy Vitamin D and Vitamin B12 Levels:

🥤 Hydration: Drink enough water daily to support proper digestion and vitamin absorption.

🌞 Exposure to the sun: Get regular morning sunlight for natural vitamin D synthesis.  Avoid smoking and limit alcohol, as these can interfere with both vitamin D metabolism and vitamin B12 absorption.

🏃 Activity (Exercise): Regular physical activity, especially outdoors, supports vitamin D production and may enhance B12 metabolism.

🍔 Lifestyle (Balanced Diet): Include vitamin D-rich foods (like eggs, mushrooms, salmon, fortified milk) and vitamin B12-rich foods (like meat, fish, dairy, or fortified cereals for vegetarians/vegans).

👩‍⚕️ Talk to Your Doctor (Consultation): Avoid self-supplementation.  Consult your doctor for proper guidance on vitamin D or B12 levels and safe supplementation if needed.

🚭 Habits (Smoking and Alcohol): These habits can interfere with the absorption and activation of vitamins B12 and D.

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What does Vitamin D (25-Hydroxy) & Vitamin B12 measure?

Contains 2 tests

The Vitamin D (25-Hydroxy) & Vitamin B12 test is tailored to determine the levels of two important vitamins in your body: vitamin D (25-OH) and vitamin B12. Vitamin D is an essential nutrient that can be synthesized in the body upon healthy exposure to sunlight or absorbed from dietary sources. On the other hand, vitamin B12 is vital for maintaining a healthy nervous system, making red blood cells, and creating the genetic material of our cells.

This test is generally recommended as a part of routine health checkups, nutritional assessments, or to monitor vitamin levels in individuals with dietary restrictions or those who are on vitamin supplements.

The Vitamin B12 measures the amount of vitamin B12 in your blood. Vitamin B12 is important for a healthy nervous system, making red blood cells, and creating DNA in your cells. Low B12 levels are common in older adults, children, vegans or vegetarians, people with diabetes, those who had gastric bypass surgery, breastfeeding women, and people with conditions like Crohn’s disease that affect vitamin absorption. High B12 levels can occur in liver diseases, certain blood disorders, or from taking vitamin supplements. Usually, high levels from supplements are not harmful, as extra B12 is removed through urine.

What Do the Results Mean?

Pathological Factors 

Test Parameter

Interpretation (possible causes)

Low levels

  • Anaemia

  • Term pregnancy

  • Intrinsic factor deficiency

  • Partial gastrectomy/ileal damage

  • Celiac disease

  • Parasitic infestation

  • Pancreatic deficiency

  • Treated epilepsy

  • Hemodialysis 

High levels

  • Renal failure

  • Hepatocellular disorders

  • Myeloproliferative disorders and at times with excess

  • Supplementation of vitamin pills.

Key: ⇧: Indicates High Levels , ⇩: Indicates Low Levels

Physiological Factors

🧒 Age: Older adults may absorb less vitamin B12 due to lower stomach acid.

🫄 Pregnancy: Higher nutritional needs during pregnancy can temporarily lower B12 levels.

💊 Hormones: Hormone therapy or birth control pills can slightly affect B12 absorption.

Lifestyle Factors

🥗 Diet: Vegetarian or vegan diets without animal products may lead to low B12 intake.

🍷 Alcohol: Excessive alcohol can reduce B12 absorption and storage in the liver.

🚬 Smoking: Smoking cigarettes may reduce vitamin B12 activity.

Disclaimer: This information is for educational purposes only and should not replace professional medical advice, diagnosis, or treatment. Always consult your healthcare provider for personalized guidance.

What Do Normal Vitamin B12 Levels Mean?

Normal B12 levels show that your body has enough vitamin B12 for healthy red blood cells, nerve function, and metabolism. This helps maintain energy, mood, and brain health.

What Do High Vitamin B12 Levels Mean? 

High B12 levels are uncommon. They can happen due to too many supplements or health conditions like liver or kidney problems, or certain blood disorders. Your doctor may order extra tests to find the cause.

What to Do If Your Vitamin B12 Is High? 

Do not stop supplements suddenly. Your doctor will review your medical history, medications, and may suggest tests to check liver, kidney, or blood health. Often, adjusting supplements is enough to bring levels back to normal.

What Do Low Levels of Vitamin B12 Indicate?

Low vitamin B12 levels suggest a deficiency, which can lead to anemia, fatigue, and nerve damage if left untreated. Common causes include:

  • Poor dietary intake (especially in vegetarians or vegans)
  • Malabsorption due to gastric or intestinal issues
  • Pernicious anemia
  • Certain medications (e.g., metformin, proton pump inhibitors)
  • Treatment usually involves vitamin B12 supplements or injections based on deficiency severity.

Can Vitamin B12 Results Be Inaccurate?

Yes. While the test is reliable, results may sometimes be inaccurate due to recent vitamin B12 injections or supplements, pregnancy or hormonal therapy, liver or kidney diseases affecting vitamin metabolism, or laboratory variations in sample handling.

What Factors Can Affect Vitamin B12 Results?

Though it is a reliable test, several factors can influence accuracy:

  • Recent intake of vitamin B12 supplements or injections
  • Liver or kidney disorders
  • Alcohol consumption or chronic illness
  • Use of medications like metformin or oral contraceptives
  • Pregnancy or hormonal therapy

What Follow-Up Tests May Be Required?

Based on your Vitamin B12 results, your doctor may recommend additional tests to get a clearer picture of your health or to monitor possible complications. These may include: 

Table: Tests Commonly Ordered Alongside Vitamin B12

Test Name

Primary Purpose

Folate (Vitamin B9) Test

Check folate levels, since low folate can also cause anemia similar to B12 deficiency.

Homocysteine Test

Measure homocysteine in the blood, which can rise when B12 is low.

Methylmalonic Acid (MMA) Test

Detect early B12 deficiency by measuring MMA, which increases when B12 is low.

Complete Blood Count (CBC) Test

Provide details about red blood cells and hemoglobin to indicate type of anemia.

Intrinsic Factor Antibody Test

Detects autoimmune causes of B12 deficiency.

Gastrointestinal Absorption Tests

Checks if the stomach or intestines are absorbing B12 properly, e.g., after gastric surgery or in conditions like Crohn’s disease.

How Does the Vitamin B12 Test Differ from Other Blood Tests?

It is important to understand how the Vitamin B12 test differs from other common blood tests:

Complete Blood Count (CBC): Checks overall blood health and red blood cells. CBC can suggest anemia but doesn’t show the cause. Vitamin B12 specifically measures the nutrient needed to produce healthy red blood cells.

Folate (Vitamin B9) Test: Folate levels also affect red blood cell production. Unlike B12, folate deficiency alone does not directly indicate nerve or brain-related issues.

Iron Studies: Tests like serum iron or ferritin detect iron-related anemia. Vitamin B12 deficiency anemia occurs even if iron levels are normal.

Homocysteine & Methylmalonic Acid (MMA): These are functional tests that rise when B12 is low. The Vitamin B12 test directly measures the B12 level, making it the first step to detect deficiency.

Know more about Vitamin B12

The Vitamin D (25-Hydroxy) test measures the levels of vitamin D in the body. Vitamin D is an essential nutrient that can be synthesized in the body upon healthy exposure to sunlight or absorbed from dietary sources. It majorly exists in two forms: vitamin D2 (ergocalciferol) and vitamin D3 (cholecalciferol). Vitamin D2 is present in plants, such as yeast or mushrooms, and is available as a supplement in fortified foods, and vitamin D3 is found in foods like cheese, green vegetables, mushrooms, egg yolks, and fatty fish.

Both forms of vitamin D need to be converted in the liver and kidneys before the body can use them effectively. This test shows the total level of vitamin D (D2 + D3) circulating in the body and guides your doctor in recommending the right treatment or lifestyle changes if needed.

Know more about Vitamin D (25-Hydroxy)

FAQs related to Vitamin D (25-Hydroxy) & Vitamin B12 in Jaipur

The Vitamin D (25-Hydroxy) & Vitamin B12 test can be done at any time of the day as no fasting is required for getting tested.
A phlebotomist (a trained professional to perform blood draws) will clean your skin using an antiseptic alcohol cotton swab or wipe and take blood samples from your vein using a needle. The blood sample will be stored safely and transported to the laboratory for analysis.
No, the Vitamin D (25-Hydroxy) & Vitamin B12 test is generally a safe procedure. While rare, bruising, bleeding, and infection at the puncture site may occur. In very uncommon cases, there can be swelling of the vein after the blood withdrawal.
Yes, Tata 1mg Labs offers the Vitamin D (25-Hydroxy) & Vitamin B12 test in Jaipur near you. The test is easy to book, is affordable, and comes with a comprehensible report.
Yes, Tata 1mg Labs offers a home sample collection facility for the Vitamin D (25-Hydroxy) & Vitamin B12 test in Jaipur. You can also select the date and time for sample collection and schedule the package at your convenience.
If you want to schedule the Vitamin D (25-Hydroxy) & Vitamin B12 test with Tata 1mg Labs in Jaipur, the procedure is fairly easy and hassle-free. Follow these steps to book a test: 1). Visit the Lab Tests section on the Tata 1mg website. 2). Add the test you want in the cart. 3). Pick a time and date slot according to your convenience; however, do consider the necessary prerequisites while scheduling a test. 4). You will receive a notification with all the details once the test is scheduled.
Tata 1mg Labs is a pocket-friendly platform for booking lab tests. In Jaipur, the Vitamin D (25-Hydroxy) & Vitamin B12 test is available at an affordable price of Rs. 649.
At Tata 1mg Labs, we understand the importance of timely results. We strive to provide fast turnaround times for test results, allowing you to promptly get the information you need. On average, we take 12-15 hours to provide the reports of the Vitamin D (25-Hydroxy) & Vitamin B12 test in Jaipur.
Content created by
Written by:
Shweta Prakash
MSc, BSc
Reviewed by:
Dr. Naziya Maner
DNB, DCP
Want to know moreRead our editorial policy
Last Updated27th Nov 2025

Vitamin D (25-Hydroxy) & Vitamin B12 test price for other cities

Price inBangaloreRs. 1149
Price inHyderabadRs. 649
Price inPuneRs. 1149
Price inMumbaiRs. 1149
Price inNew DelhiRs. 1149

How does home sample collection work?

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Contains 2 tests
Vitamin B12
Vitamin D (25-Hydroxy)