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Vitamin D (25-Hydroxy)

Vitamin D (25-Hydroxy) near me in Nashik

Also referred as
Calcidiol
Vit D (25-OH)
18,450+ booked recently
For men & women
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The Vitamin D (25-Hydroxy) test is tailored to measure vitamin D levels in the body and is available at an affordable price in Nashik with Tata 1mg labs. Vitamin D is a fat-soluble essential nutrient that helps the body maintain strong bones & teeth and a healthy immune system. It can be obtained from healthy exposure to sunlight and dietary sources. 

Your doctor may suggest the Vitamin D (25-Hydroxy) test if they notice health issues associated with low vitamin D levels like an increased risk of fractures, muscle weakness and lethargy, dysfunction of the immune system, and increased risk of chronic diseases like diabetes. This test also monitors the treatment response in people receiving medication for abnormal vitamin D levels. 

It is generally recommended to stop taking vitamin D supplements before taking Vitamin D (25-Hydroxy) test, as they may affect the accuracy of the test results. However, you may consume a regular diet before taking the test.

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Understanding Vitamin D (25-Hydroxy)

Vitamin D is an essential fat-soluble nutrient. It exists in two primary forms:

Vitamin D3 (Cholecalciferol): derived from animal sources, supplements, and made by the skin when you are in sunlight.

Vitamin D2 (Ergocalciferol): derived from plant sources and supplements.

Both Vitamin D2 and D3 perform similar functions in the body and are converted in the liver to 25-hydroxy vitamin D2 or D3, respectively. The Vitamin D (25-Hydroxy) test measures the total vitamin D in your blood (D2 + D3) but does not differentiate between the two forms.

As per an NCBI (National Center for Biotechnology Information) study, vitamin D deficiency is highly prevalent across all age groups in India. Insufficient vitamin D levels can increase susceptibility to the following conditions:

  • Fractures
  • Weakened muscles
  • Fatigue
  • Compromised immune function
  • Higher likelihood of chronic illnesses like diabetes

The Vitamin D (25-Hydroxy) test is done to check if you have health problems that affect how your body uses vitamin D, such as weak bones (osteoporosis), or kidney or liver issues. It is also done if there are risk factors like insufficient sunlight exposure or a poor diet. It is also used for the diagnosis of causes of rickets and osteomalacia. Additionally, this test is done to monitor vitamin D levels in people who are taking supplements, to ensure their levels stay within a healthy range and are not too low or too high.

Usually, no special preparation is required for this test. You can get this test at any time of the day at your convenience. However, let the doctor know about all the medications being taken, as some can affect the test results.

Always consult your doctor to understand what your results mean. They can guide you with the right treatment, lifestyle changes, or medication if needed.

What are the symptoms or conditions that may require the Vitamin D (25-Hydroxy) test?

The Vitamin D (25-Hydroxy) test is needed if: 

  • You have frequent bone or muscle pain.
  • You feel persistent fatigue or weakness.
  • You suffer from frequent infections.
  • You have brittle bones or fractures.
  • You are at risk due to low sun exposure or a poor diet.

When Would I Need the Vitamin D (25-Hydroxy) Test?

You may need the Vitamin D (25-Hydroxy) test:

  • To detect or confirm vitamin D deficiency or excess.
  • If you have weak bones, muscle pain, or fatigue.
  • To monitor vitamin D supplementation therapy.
  • To assess calcium absorption issues.
  • If your doctor suspects osteoporosis or bone metabolism disorders.

How Should I Prepare for the Vitamin D (25-Hydroxy) Test?

Do I need to fast or stop any medications before the Vitamin D (25-Hydroxy) test?

No, fasting is not required before the Vitamin D (25-Hydroxy) test. You can eat and drink normally before the test. However, it is essential to inform your doctor about any medications or supplements you are taking, as certain medications may interact with test results. Your doctor will guide you if any temporary adjustments are needed before the test.

How is the Vitamin D (25-Hydroxy) done?

  • The Vitamin D (25-Hydroxy) is done by collecting a small amount of blood from a vein, usually in the arm. 
  • The procedure is quick, minimally invasive, and generally causes only slight discomfort.

What happens during the Vitamin D (25-Hydroxy) test?

  • You will be seated comfortably with your arm supported on a flat surface or armrest.
  • A tourniquet (elastic band) is tied around your upper arm to make the veins more visible and easier to locate.
  • The phlebotomist identifies a suitable vein for blood collection.
  • The puncture site is then cleaned thoroughly with an alcohol swab to reduce the risk of infection.
  • A thin, sterile needle is gently inserted into the vein, and a small amount of blood is collected into a test tube.

What happens after the Vitamin D (25-Hydroxy) test?

  • Once enough blood is collected, the tourniquet is released and the needle is removed.
  • A cotton ball or gauze is placed on the site, followed by a small bandage or dressing to stop any bleeding.
  • The blood sample is carefully labeled with your details and sent to the laboratory for analysis.
  • You can resume your daily routine after the test is done.

Is the Vitamin D (25-Hydroxy) test painful?

No, the Vitamin D (25-Hydroxy) test is generally safe and painless. However, you may feel slight discomfort during blood sample collection, but the chances of serious complications are extremely rare.

How Long Does the Vitamin D (25-Hydroxy) Test Take?

The procedure is quick, simple, and safe. It requires only a small blood sample and is completed within a short duration.

When Will I Get the Results?

The availability of your test report may vary depending on your location and the laboratory where the test is conducted. In general, Tata 1mg provides the results within the stipulated time after sample collection.

When Should I Consult a Doctor After the Test?

  • Your vitamin D levels are outside the normal range.
  • You have symptoms like bone pain, weakness, or frequent illness.
  • You are on supplements or medications that affect vitamin D levels.
  • Your doctor will help to identify the causes and recommend appropriate management.

Are There Any Risks Associated with the Vitamin D (25-Hydroxy) Test?

The Vitamin D (25-Hydroxy) test is safe, with only minimal and short-lasting effects like:

  • Small bruise, pinch, or slight tenderness at the puncture site
  • Occasional lightheadedness if you are sensitive to needles or blood
  • Rarely, infection or blood vessel injury

Note: If you have a bleeding disorder or take blood thinners, inform the phlebotomist beforehand.

Lifestyle Tips to Support Healthy Vitamin D Levels 

☀️ Heliotherapy (Sunlight Exposure): Spend some time outdoors, under the sun (preferably in the morning), to help your body naturally produce vitamin D.

🥙 Eat (Diet): Include vitamin D-rich foods such as eggs, mushrooms, salmon, fortified milk, and orange juice.

🏃 Activity (Exercise): Engaging in regular physical activity, especially outdoors, can support better vitamin D synthesis and bone health.

😓 Lifestyle (Stress): Manage stress through relaxation techniques or yoga, as stress can indirectly impact bone health.

👨‍⚕️ Talk to Your Doctor (Consultation): Avoid self-medication and taking high doses of vitamin D supplements without a doctor's advice.

🚭 Habits (Alcohol & Smoking): Limit alcohol and quit smoking, as these can affect vitamin D metabolism and bone strength.

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What does Vitamin D (25-Hydroxy) measure?

The Vitamin D (25-Hydroxy) test measures the levels of vitamin D in the body. Vitamin D is an essential nutrient that can be synthesized in the body upon healthy exposure to sunlight or absorbed from dietary sources. It exists in two forms: vitamin D2 (ergocalciferol) and vitamin D3 (cholecalciferol). Vitamin D2 is present in plants, such as yeast or mushrooms, and is available as a supplement in fortified foods. Vitamin D3 is found in foods like cheese, green vegetables, mushrooms, egg yolks, and fatty fish.

Both forms of vitamin D need to be converted in the liver and kidneys before the body can use them effectively. This test shows the total level of vitamin D (D2 + D3) circulating in the body and guides your doctor in recommending the right treatment or lifestyle changes if needed.

What Do the Results Mean?

Pathological Factors 

Test Parameter

Interpretation (possible causes)

Normal / Optimal

  • Sufficiency

Possible causes: HPT (Hyperparathyroidism)

Humoral hypercalcemia of malignancy

Low

  • Deficiency 

Possible causes can be renal failure, hyperphosphatemia, vitamin D–dependent rickets, types 1 and 2

Borderline / Moderate Low

  • Insufficiency (due to inadequate dietary intake or low sunlight exposure)

High / Excess

  • Hypervitaminosis

Possible causes can be sarcoidosis, non-Hodgkin lymphoma 

Key: ⇧: Indicates High Levels , ⇩: Indicates Low Levels

Physiological Factors

🧒 Age: Older adults synthesize less vitamin D from sunlight primarily because of age-related changes in their skin.

🫄 Pregnancy: Vitamin D demand increases during pregnancy for fetal bone development.

💊 Hormonal Influence: Hormonal changes or contraceptive use may alter vitamin D metabolism.

Lifestyle Factors

🌤️ Sun Exposure: Limited sunlight reduces vitamin D synthesis.

🥗 Diet: Low intake of vitamin D-rich foods (like fish, egg yolks, or fortified dairy) can cause deficiency.

🍷 Alcohol Consumption: Chronic alcohol use affects liver function, impacting vitamin D metabolism.

🚬 Smoking: Smoking is closely associated with reduced bone density and altered vitamin D activity.

Disclaimer: This information is for educational purposes only and should not replace professional medical advice, diagnosis, or treatment. Always consult your healthcare provider for personalized guidance.

What Do Normal Vitamin D (25-Hydroxy) Levels Mean?

Normal vitamin D levels indicate your body has sufficient vitamin D for bone health, muscle strength, and immune function.

What Do High Levels of Vitamin D (25-Hydroxy) Indicate?

High vitamin D levels may occur due to excessive supplementation or conditions like sarcoidosis or hyperparathyroidism. Persistently high levels can cause vitamin D toxicity, leading to high blood calcium (hypercalcemia), which may result in nausea, vomiting, muscle weakness, and, in severe cases, kidney stones or kidney damage.

What to Do if Your Vitamin D (25-Hydroxy) Is Higher Than Normal?

If your Vitamin D (25-Hydroxy) level is high, consult your doctor for further guidance. They may review your dosage, identify causes of high calcium like excess supplement intake, or check for conditions such as hypercalcemia. Do not stop or adjust supplements without your doctor’s advice.

What Do Low Levels of Vitamin D (25-Hydroxy) Indicate?

Low levels suggest deficiency, which may cause weak bones, muscle pain, or fatigue. Common causes include:

  • Lack of sunlight exposure
  • Poor dietary intake
  • Malabsorption syndromes (e.g., celiac disease)
  • Obesity or liver/kidney disease

Treatment involves lifestyle changes, dietary improvement, or vitamin D supplements.

Can Vitamin D (25-Hydroxy) Results Be Inaccurate?

Yes. Results may vary due to recent supplement intake, laboratory variations, or conditions affecting vitamin D metabolism, such as liver or kidney disease. In some cases, additional tests may be advised to confirm the deficiency.

What Factors Can Affect Vitamin D (25-Hydroxy) Results?

Though it is a reliable test, some circumstances can influence accuracy:

  • Recent vitamin D or calcium supplement intake
  • Limited sun exposure
  • Chronic illness or liver/kidney disorders
  • Malabsorption syndromes
  • Obesity or hormonal imbalances

FAQs related to Vitamin D (25-Hydroxy) in Nashik

This test is done to measure the amount of vitamin D in individuals with symptoms such as bone deformities, dysfunction of the immune system, muscle cramps, and dental abnormalities. It is also used to monitor treatment response in individuals who are taking vitamin D supplements.
The Vitamin D (25-Hydroxy) test can be done at any time of the day.  However, you should stop taking vitamin D supplements before the test to get accurate results.
No, usually, there are no risks associated with this test.  The blood sample is withdrawn with the help of a needle, and the process is fairly fast and relatively painless.
Yes. In Nashik, you can schedule the vitamin D test near you with Tata 1mg Labs. The test is easy to book, cost-effective, and available with a comprehensive report.
Yes, Tata 1mg Labs provides a home sample collection facility for the vitamin D test in Nashik. You can choose the date and time of sample collection as per your schedule and convenience.
The booking process for the vitamin D with Tata 1mg Labs in Nashik is straightforward and hassle-free. Follow these steps to schedule a test online: 1). Go to the Lab Tests section on the Tata 1mg website. 2). Add your preferred test to the shopping cart. 3). Choose a time and day that works for you as long as you also take the essential prerequisites into account. 4). A confirmation notification will be sent to you with all the details once the booking is complete.
Tata 1mg Labs offers all tests at an affordable price. The vitamin D test is priced at Rs. 308 in Nashik.
We at Tata 1mg Labs recognize the value of prompt results. We strive to deliver test results with quick turnaround times so you can receive the information promptly. The reports of the vitamin D test in Nashik are often provided in 18-24 hours.

Vitamin D (25-Hydroxy) test price for other cities

Price inBangaloreRs. 649
Price inKolkataRs. 649
Price inHyderabadRs. 308
Price inNew DelhiRs. 499
Price inMumbaiRs. 499

How does home sample collection work?

References

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    Aparna P, Muthathal S, Nongkynrih B, Gupta SK. Vitamin D deficiency in India. J Family Med Prim Care. 2018 Mar-Apr;7(2):324-330. [Accessed 03 Nov. 2025]. Available from:
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Vitamin D (25-Hydroxy)