How can we help?

Home

Hemoglobin HPLC / Electrophoresis

Also referred as

Hb Electrophoresis

Abnormal Hb studies

Hemoglobin variant analysis

4,525+ booked recently

For men & women

Earliest reports in

72 hours

Contains

1 test

Know more about this test

The Hemoglobin HPLC / Electrophoresis test is a blood test used to check for normal and abnormal types of hemoglobin in the blood. This test helps detect and monitor blood disorders like thalassemia and sickle cell disease.

Samples required

Blood

Find out

Why is this test booked?

Preparation for this test

Sample Collection

Who will collect your samples?

Understanding Hemoglobin HPLC / Electrophoresis

Hemoglobin (Hb) is a protein in red blood cells that carries oxygen to the body’s organs and tissues. The Hemoglobin HPLC / Electrophoresis test gives a detailed report of the different types of hemoglobin, such as HbA (normal), HbF (fetal hemoglobin), and other abnormal types like HbD. This test is useful in spotting disorders that are linked to abnormal hemoglobin. It also helps doctors check how well treatment is working in people who are receiving blood transfusions or medication.

The doctor may advise this test if a blood disorder like thalassemia or sickle cell disease is suspected. Common signs include dizziness, weakness, pale skin, cold hands and feet, frequent infections, shortness of breath, fast heartbeat, or weight loss. The test may also be suggested if there is a family history of blood disorders, unexplained anemia, or abnormal results in other blood tests.

Fasting is not required for this test. Eat and drink as per the daily routine. Inform the doctor of any recent blood transfusion, as it may affect the test results.

The normal ranges of results can vary based on age, gender, health history, and the lab that does the test. The doctor will explain the results by looking at other tests like CBC, family history, and overall health. Based on the findings, the doctor will decide if any treatment or medication is needed.

Test result ranges are approximate and vary depending on age, gender, health history, and methodology used by the laboratories. Talk to the doctor about specific test results. The doctor may consider several additional factors while interpreting the significance of abnormal hemoglobin, including family history, the results of CBC, and more. Based on the test results, the doctor decides whether or not medication will be required to manage the condition and formulate an overall treatment plan.

What does Hemoglobin HPLC / Electrophoresis measure?

The Hemoglobin HPLC / Electrophoresis test identifies and measures different types of hemoglobin in the blood to help diagnose and keep track of certain blood disorders. These types include: adult type (HbA2), fetal type (HbF), hemoglobin S (HbS), hemoglobin C (HbC), and hemoglobin E (HbE), among others.

Normal hemoglobin types:

Hemoglobin A (HbA): The main type of hemoglobin in healthy adults.
Hemoglobin F (HbF): Found in unborn babies and newborns. It is slowly replaced by HbA after birth.

If the levels of HbA or HbF are not normal, it may indicate certain types of anemia.

Abnormal hemoglobin types:

Hemoglobin S (HbS): Found in people with sickle cell anemia. It causes red blood cells to become stiff and sickle-shaped, leading to pain and other health problems.
Hemoglobin C (HbC): Linked to a condition called hemolytic anemia, where red blood cells break down faster than normal.
Hemoglobin E (HbE): Common in people from Southeast Asia. It may cause mild anemia or sometimes no symptoms at all.
Hemoglobin D (HbD): Causes mild hemolytic anemia and mild to moderate spleen enlargement. The Hb D Punjab variant is most commonly seen in people from Gujarat and among Sikhs of Punjab.

Frequently booked together

FAQs related to Hemoglobin HPLC / Electrophoresis

Why do I need the Hemoglobin HPLC / Electrophoresis test?

You will need this test if your physician suspects you of having a blood disorder like thalassemia or sickle cell disease, or if a member of your family has such a disorder.

How is the Hemoglobin HPLC / Electrophoresis test performed?

A sample of blood is drawn from your arm and poured into a test tube. This sample is analyzed in the laboratory. This test divides the various types of hemoglobin and compares them with a standard sample to provide precise results.

Is there any risk associated with this est?

The test is absolutely safe. You might feel a bit sore or get a minor bruise where the needle is put in, but severe issues such as infection or excessive bleeding are uncommon.

What might affect my test results?

Factors such as your diet, age, gender, smoking, altitude (where you reside), some medicines, and illness may influence the results.

Can this test be used to screen family members for hemoglobin disorders?

Yes, in case of a familial history of hemoglobin disorders, this test can be used to screen other members of the family. It can also assist in family planning.

Is this test done for newborns?

Yes, it can be performed as part of newborn screening to identify blood disorders at an early stage and initiate treatment in a timely manner.

What is hemoglobinopathy?

Hemoglobinopathy is a term for a family of inherited disorders in which the body produces abnormal hemoglobin. Such diseases are inherited from parents to offspring.

What are the common examples of hemoglobinopathies?

Hemoglobin C disease, hemoglobin S-C disease, sickle cell anemia, and thalassemia are common hemoglobinopathies.

What are the complications of thalassemia?

Thalassemia can result in infections, iron overload, bone changes, enlarged spleen, retarded growth, and cardiac complications.

How do I increase my hemoglobin levels if it is low?

Talk to your doctor if your hemoglobin is low. Eating iron-rich foods, taking iron supplements, and adding vitamin C to your diet can help improve your hemoglobin levels.

Content created by

Want to know moreRead our editorial policy

Last Updated

7th Oct 2025