HLA B-27, Flowcytometry
What is HLA B-27, Flowcytometry?
HLA B-27 refers to Human leukocyte antigen B-27. This test is done to determine the presence of HLA B-27 in the blood. Along with that, it helps to assess the presence of any autoimmune disorder associated with HLA B-27.
Why is HLA B-27, Flowcytometry done?
The HLA B-27 test is done:
In case of symptoms of arthritis such as chronic joint pain, stiffness, and inflammation in the lower back, hips, neck, and chest
In the case of Uveitis (painful inflammation of eyes) especially in males and of the early 30s age group.
What does HLA B-27, Flowcytometry Measure?
HLA B-27 test determines the presence or absence of human leukocyte antigen which is present on the surface of white blood cells. The HLA B-27 also refers to the gene which codes for the HLA B-27 protein.
HLA B-27 are proteins which help the immune system to differentiate the body’s own cells from other foreign cells. Every person has an inherited combination of HLA genes which codes for many antigens which are present on the surfaces of their cells. This HLA combination is distinctive for each person and is based on the presence or absence of each antigen.
People with HLA B-27 have increased chances of developing autoimmune diseases like ankylosing spondylitis, rheumatoid arthritis, reactive arthritis, and isolated acute anterior uveitis. HLA B-27 is also said to be linked with inflammatory bowel disease and other chronic conditions.
Interpreting HLA B-27, Flowcytometry results
No expression of HLA B-27
Grey zone (within 10 channels of cutoff)
Expression of HLA B-27 detected
Anti-HLA-B27 antibody cross reacts with several other antigens of HLA-B group specially HLA-B7 leading to false-positive results*