Accurex Accu Test Vitamin D Test Kit is a rapid chromatographic immunoassay for the semi-quantitative detection of vitamin D levels in human blood/serum. This assay provides only a preliminary screening test result. A more specific alternative chemical method must be used to obtain a confirmed analytical result. The performance characteristics of this assay have not been established in a pediatric population.
Uses:
This kit is used for the detection of vitamin D levels in human blood/serum.
Product Specifications and Features:
It is based on the principle of a competitive enzyme immunoassay
The assay is based on the competition for 25-OH Vitamin D present in the blood/serum sample and vitamin D on the test line for a fixed number of the antibody-gold conjugate
Depending upon the vitamin D concentration in blood/serum, free antibody-gold conjugate molecules will bind to Vitamin D on the test strip and show a coloured line in the test line zone
A control line is present in the test window to work as procedural control
Read test results for 10 minutes as required
Results may deteriorate and not be consistent after 10 minutes
In order to avoid variable results, mix the blood sample thoroughly
A serum sample subjected to repeated freeze-thaw cycles may give variable results
Directions For Use:
Remove the cassette from the sealed pouch and place it on a hard flat surface with the view window facing up (use the cassette as soon as possible)
Using a micropipette, add 10 µl of whole blood or 5ul of serum sample directly into the rectangular specimen well (marked with Red drop) of the cassette
Add 3 full drops of buffer into the square buffer well of the cassette (do not move the cassette after addition of buffer)
Let the cassette sit for 10 minutes, and read your results immediately
Results may deteriorate after the 10-minute mark
Safety Information:
For in vitro diagnostic use only
The test is designed for use with human blood/serum only
Reagents must be at room temperature (8⁰C – 30 ⁰C)
Do not use if the test device packaging is open or damaged
Do not use the product beyond the expiration date
Handle all specimens as potentially infectious. Proper handling and disposal methods should be used according to good laboratory practices
