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Written by
Dr. Shreya Gupta
BDS, MDS - Oral and Maxillofacial Pathology
Reviewed by
Dr. Ashish Ranjan
MBBS, MD (Pharmacology)
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PFT with Diffusion Study (DLCO)

Also known as Pulmonary Function Test with Diffusion Study
This test is for
Male, Female
Test Preparation
  1. Take your daily medications before your test unless your doctor tells you not to.
  2. If you use a short-acting inhaler, try not to use it for 6 to 8 hours before the test. If you have to use it, let your doctor know before the test.
  3. Avoid smoking for at least 6 hours before the test.
  4. Don’t drink alcohol at least 4 hours before the test.
  5. Don’t eat a large meal at least 2 hours before the test.
  6. Don’t exercise for at least 30 minutes before the test.
  7. It is advised to wear loose clothing that does not squeeze the chest or interfere with breathing.

Understanding PFT with Diffusion Study (DLCO)


What is PFT with Diffusion Study (DLCO)?

The Pulmonary Function Test (PFT) with Diffusion Study, also known as DLCO (Diffusing Capacity of the Lungs for Carbon Monoxide),is a simple and safe breathing test that checks how well the lungs are working—specifically, how well gases like oxygen pass from the lungs into the blood. During the procedure, the individual breathes in a special gas mixture containing nitrogen, oxygen, a very small amount of carbon monoxide (at a harmless level), and a tracer gas. As the gas is inhaled, the lungs absorb some of the carbon monoxide. The individual then exhales into a machine, which measures the amount of carbon monoxide remaining in the breath. This measurement helps determine how efficiently the lungs are transferring gases, helping diagnose lung conditions such as chronic obstructive pulmonary disease (COPD), pulmonary fibrosis or emphysema and can also track how well treatments are working.

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