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The Testosterone Total test measures the level of testosterone hormone available in the blood. This includes two forms of testosterone: free testosterone and bound testosterone. Bound testosterone is attached to proteins, mainly to sex hormone-binding globulin (SHBG) and albumin. It cannot enter the body's cells or directly affect tissues and organs. Instead, it acts as a store of testosterone in the blood and can be released when the body needs it. Free testosterone is not attached to any proteins and is the active form. It can easily transport to various tissues, and affects key functions like sex drive, muscle growth, bone strength, and energy levels.

An HbA1c (Glycosylated Hemoglobin) test precisely measures the percentage of sugar-coated or glycated hemoglobin in your blood. The test results represent the proportion of hemoglobin in your blood that has been glycated. 

Hemoglobin, a vital protein found in red blood cells, is responsible for transporting oxygen throughout the body. Hemoglobin A is the most abundant form of hemoglobin, and when blood sugar levels increase, a higher proportion of hemoglobin A becomes glycated. As red blood cells have a lifespan of approximately 120 days, the sugar molecules remain attached to the hemoglobin for the duration of the cell's life. Consequently, the HbA1c test offers insight into your average blood sugar levels over the past 8 to 12 weeks.

The Insulin, Fasting test evaluates the body's ability to regulate blood sugar levels by measuring the amount of insulin in the blood after an overnight fast. Glucose is a source of energy that helps the body perform daily activities. Your body gets glucose from carbohydrates that are consumed in the diet. These carbohydrates are digested and converted into simple sugar (glucose). The cells then absorb the required amount of glucose to produce energy, and the remaining amount is stored in other forms, like fats. The insulin hormone helps the cells to uptake glucose from the blood for utilization and storage. Thus, it is necessary to maintain insulin levels in your body.

The hormonal profile measures the amount of hormones, Follicle-Stimulating Hormone (FSH), Luteinizing Hormone (LH), Prolactin, and Thyroid-Stimulating Hormone (TSH), to determine your reproductive health. This test is performed to address health issues such as delayed puberty, irregular menstruation, abnormal breast milk discharge, loss of libido, erectile dysfunction, and problems related to fertility.

These hormone levels are usually low at a young age, but the brain starts producing gonadotropin-releasing hormone (GnRH) as adolescence approaches. GnRH is responsible for initiating the changes in puberty and signaling the pituitary gland to release FSH and LH into your blood.