What is the HPV DNA Detection and Genotyping by PCR test?
The HPV DNA Detection and Genotyping by PCR test is an advanced molecular test designed to identify the genetic material (DNA) of Human Papillomavirus in cervical samples. Unlike routine screening methods, this test not only confirms the presence of HPV infection but also determines the exact HPV genotype, especially high-risk strains such as HPV-16 and HPV-18, which are responsible for the majority of cervical cancer cases.
The test uses the PCR technique, which amplifies even very small amounts of viral DNA, making it highly sensitive and accurate, even in early or asymptomatic stages of infection.
What is the process flow for HPV DNA Detection and Genotyping by PCR test?
HPV DNA PCR Test – Process Overview
1. Book Your Test
- You select and book the HPV DNA test through the Tata 1mg platform.
2. Kit Delivery & Support
- Pragmatech delivers the CERVICHECK™ kit to your home. CERVICHECK™ self-sampling kit would take 4–5 working days (96 to 120 hours) to deliver via courier. CERVICHECK™ is India’s first CDSCO-approved at-home self-sampling kit for cervical cancer screening
- Their team gets in touch for simple instructions and guidance.
3. Collect Your Sample
- Use the CERVICHECK™ kit to collect your sample.
- Fill in the Customer Information Form (CIF) included in the kit.
4. Ready for Pickup
- After collecting your sample, inform the Pragmatech Team.
- Our team schedules a pickup from your home.
5. Sample Transport
- Your sample is sent to the Tata 1mg Lab (NRL, Okhla) for testing.
- Sample stays safe and stable during transport.
6. Lab Testing
- Our lab experts process the sample and run the HPV DNA PCR test.
- The sample is checked for HPV and its types, as per the doctor’s recommendation.
7. Results and Data
- Your results are shared securely once ready.
- All data is managed carefully by Tata 1mg and Pragmatech for your privacy.
Don’ts:
1. The CERVICHECK™ Self Sampling kit should be only used for the intended purpose, i.e., for cervical sampling
2. Do not use if you have undergone a total hysterectomy.
3. Do not use if you are pregnant.
4. Do not use if you are using any vaginal medications
Dos:
1. Read the instructions carefully before proceeding.
2. Collect the sample within 5 days of receiving the kit and inform for sample pickup.
3. Refrain intercourse 24 hours before sampling
Why is HPV genotyping important?
HPV genotyping is vital for precise risk stratification because it identifies the specific strains (mainly HPV 16 and 18) that carry the highest oncogenic potential.
Who should consider the HPV DNA Detection and Genotyping by PCR test?
Every healthy woman between 25 – 65 years should get themselves screened periodically for HPV.
What does the HPV DNA Detection and Genotyping by PCR test results mean?
If the report is negative/ not detected it means NO high-risk viral DNA was found in your sample and you are at very low risk of developing cervical cancer in the next 5-10 years. If the report is Positive it means that you have an HPV infection. However, this does NOT necessarily mean that you have cancer. You are requested to meet a gynaecologist and further tests are to be done as per gynaec’s suggestion.
When to consult a gynecologist?
In case your HPV report is Positive, then you should consult a gynaecologist.
How is this test different from a Pap Smear?
A PAP test identifies if cancer or any abnormality is present in the cervix or not. It is a microscopic analysis performed on sample collected by the gynaecologist. It does not detect HPV and hence it does not detect early risk to cervical cancer.
What are the possible risks or discomforts of HPV DNA Detection and Genotyping by PCR?
The CERVICHECK self sampling kit has been tested for its safety and accuracy when used as per the instruction given. It has been found to be safe and accurate. With over 3000 users, no adverse event has ever been reported.
What are the limitations of the HPV DNA Detection and Genotyping by PCR test?
- A negative result does not completely rule out HPV infection, as infection may be acquired later.
- The test cannot detect cervical cell changes; abnormal cells may exist even if HPV is not detected.
- Low-risk HPV infections may resolve spontaneously, while high-risk HPV may require further testing.
- Results may be affected by incorrect sample collection or handling.
Disclaimer: This test is safe and minimally invasive when instructions are followed correctly. Mild discomfort during sample collection may occur. Do not use the kit if you are pregnant.
Note: This test is intended for screening and risk assessment purposes only. Results cannot alone diagnose cervical cancer or other conditions. Always consult a qualified healthcare professional for interpretation and follow-up.
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