AFB DNA Detection by RT-PCR, Sputum,

Also referred as

Tuberculosis DNA PCR - Qualitative

TB DNA Detection by Realtime PCR

Mycobacterium TB Complex

MOTT Test

For men & women

Earliest reports in

66 hours

Contains

2 tests

Test price:

₹2060

~~₹2290~~

10% off

More offers available

Know more about this test

The AFB DNA Detection by RT-PCR, Sputum, test is a molecular diagnostic test that detects the genetic material (DNA) of Mycobacterium tuberculosis complex (MTBC), the bacteria responsible for tuberculosis (TB), and may also detect MOTT (Mycobacteria Other Than Tuberculosis) or non-tuberculous mycobacteria (NTM). It is commonly used for the early diagnosis of pulmonary tuberculosis and other mycobacterial lung infections.

Samples required

Sputum

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Why is this test booked?

Preparation for this test

Sample Collection

Who will collect your samples?

Test conducted by

Tata 1mg Dwarka (In Partnership with Protime Labs)

PCPNDT certified

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Know more about lab

Tata 1mg Dwarka (In Partnership with Protime Labs)

PCPNDT certified

About this lab

We Protime Labs are a super specialized state of the art ,Doctors owned and run Laboratory, Having all kinds of tests done in house by the best of the machines and supervised by highly qualified Doctors . Our laboratory has the facility to undertake all kinds of blood tests , Cytopathology, Histopathology tests , along with Echocardiography, Treadmill test, Pulmonary function test, NCV, EMG, EEG . Our specialized center is run by super specialist doctors , you will be assured of best quality work ,Our doctors are also available to discuss your report to help you understand the disease process. We promote highest quality work. We promote compassion, Quality and Scientifically proven Test.

Understanding AFB DNA Detection by RT-PCR, Sputum,

Tuberculosis (TB) is an infectious disease caused by bacteria belonging to the Mycobacterium tuberculosis complex (MTBC). It most commonly affects the lungs but can also involve other organs. TB spreads through the air when a person with active pulmonary TB coughs, sneezes, or speaks. A related group of organisms, known as non-tuberculous mycobacteria (NTM) or Mycobacteria Other Than Tuberculosis (MOTT), are environmental bacteria that can cause lung and other infections, particularly in individuals with underlying lung disease or weakened immunity. Both MTBC and NTM are classified as acid-fast bacilli (AFB) due to their unique cell wall structure, which requires specialized staining methods for microscopic detection.

The AFB DNA Detection by RT-PCR, Sputum, test uses real-time PCR technology to find out the DNA of these bacteria in a sputum sample. It is especially useful for individuals with a persistent cough (>2 weeks), unexplained weight loss, fever, or other TB symptoms. It is also useful for those with close contact with an individual diagnosed with TB, individuals with weak immune systems (e.g., HIV/AIDS, diabetes, cancer patients, or those on immunosuppressive drugs), and healthcare workers or individuals living or working in high-risk settings (hospitals, prisons, homeless shelters, etc.).

It is noteworthy that the AFB DNA Detection by RT-PCR, Sputum, test may not detect TB if the bacterial load is very low or if the sample is inadequate.

Collect sputum in a sterile, leak-proof container with a screw-cap lid. Before collecting the sample, rinse your mouth with clean water. Take two to three deep breaths, then cough deeply to bring up sputum from the lungs. Ensure you collect at least 2 ml of thick, yellowish (mucopurulent) sputum. Avoid collecting only saliva or nasal secretions. As per National Tuberculosis Elimination Programme (NTEP) guidelines, testing requires two sputum samples. This may include a spot and early morning sample collected on consecutive days or two spot samples collected one hour apart for accurate diagnosis of tuberculosis (TB).

Laboratory test results may vary across laboratories. Do not self-medicate and always consult a doctor to understand the test results correctly. In some cases, repeat testing or additional confirmatory tests may be warranted.

Please note: This is an outsourced test. The sample is collected by TATA 1MG and processed at a NABL accredited partnered lab.

What does AFB DNA Detection by RT-PCR, Sputum, measure?

Contains 2 tests

The AFB DNA Detection by RT-PCR, Sputum, test measures the presence of genetic material (DNA) from Mycobacterium tuberculosis complex (MTBC), the group of bacteria that causes tuberculosis (TB). This test also helps identify MOTT (Mycobacteria Other Than Tuberculosis), which may cause opportunistic infections, especially in people with low immunity. By using RT-PCR technology, this test detects specific DNA sequences of these bacteria in the sputum sample. It helps distinguish MTBC and MOTT to find the bacteria causing the infection. The test helps clinicians rapidly identify the type of infection and initiate suitable treatment. However, results should always be interpreted along with clinical findings, imaging studies, and other laboratory tests.

Mycobacterium TB complex

MOTT

Test Information is reviewed by: Dr. Swati Gupta (Anand), MBBS, MD Lab Medicine and written by Dr. Lipika Khurana, BDS, PGDHHM . The test details are for information purpose only. Consult a doctor before taking any test. Last updated on: 28th Jun 2026

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Price in

New Delhi

Rs. 2060

FAQs related to AFB DNA Detection by RT-PCR, Sputum,

What does the AFB DNA Detection by RT-PCR, Sputum, test detect?

The AFB DNA Detection by RT-PCR, Sputum, test helps detect the genetic material (DNA) of Mycobacterium tuberculosis complex, the causative organism of tuberculosis (TB), and of MOTT (Mycobacteria Other Than Tuberculosis), bacteria that may cause other lung infections.

Who should get the AFB DNA Detection by RT-PCR, Sputum, test?

Individuals showing TB symptoms, those in contact with TB patients, or those with non-resolving respiratory infections.

Do I need to fast before the AFB DNA Detection by RT-PCR, Sputum, test?

Fasting is not required before this test. You can eat and drink normally as per your daily routine unless advised otherwise.

How is the AFB DNA Detection by RT-PCR, Sputum, test done?

The AFB DNA Detection by RT-PCR, Sputum, test is done by providing a sputum (mucus from your lungs) sample, which you will need to cough up into a sterile container. Once collected, the sample is sent to a laboratory where it undergoes testing to detect the presence of TB bacteria. If TB is confirmed, the same sample is then checked for resistance to rifampicin, a critical TB antibiotic.

Is the AFB DNA Detection by RT-PCR, Sputum, test better than a sputum smear?

Yes, the AFB DNA Detection by RT-PCR, Sputum, test is better than a sputum smear, as PCR-based tests are more sensitive and provide drug resistance details.

What is MOTT?

MOTT (Mycobacteria Other Than Tuberculosis) is also known as non-tuberculous mycobacteria (NTM). It refers to a group of mycobacteria that are distinct from the bacteria that cause tuberculosis (TB) and leprosy. These organisms are commonly found in the environment, including soil and water.

How is MOTT different from tuberculosis (TB)?

Unlike TB, which is caused by Mycobacterium tuberculosis and can spread from person to person through the air, MOTT infections are usually acquired from environmental sources such as soil and water. MOTT infections are generally not considered contagious and most often occur in individuals with underlying lung disease or weakened immune systems.

How does Tata 1mg ensure accurate lab test results?

Tata 1mg ensures accurate lab test results through certified laboratories that use advanced technology and adhere to stringent quality control measures. This commitment to high standards guarantees reliable results.

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Recommended for everyone

This package is designed with everyone’s overall health considerations in mind, offering assessments to address a wide range of wellness needs.

Package can be booked by :

Men

Women

Contains 2 tests

Mycobacterium TB complex

MOTT

Report delivery

Standard time

66 hrs

For slots after 11 AM, report will be delivered in 120 hours.

Samples required

Sputum

1 container required

Preparations

The sputum sample should preferably be collected early in the morning before eating or drinking. Before collection, rinse the mouth thoroughly with plain water and avoid using mouthwash, antiseptic gargles, or toothpaste immediately beforehand. Take 2–3 deep breaths and cough deeply from the chest to bring up sputum (phlegm) from the lungs. Avoid submitting saliva with sputum. Collect the sputum directly into the sterile, wide-mouthed container provided by the laboratory. A good-quality sputum sample is usually thick, mucoid, or purulent rather than thin and watery like saliva. Whenever possible, the sample should be collected before starting antibiotics or anti-tubercular treatment, unless otherwise advised by the treating clinician. After collection, ensure that the container is tightly closed immediately.

Why is this test booked?

To help diagnose tuberculosis (TB).

To identify non-tuberculous mycobacterial (MOTT/NTM) lung infections.

To investigate persistent cough, fever, night sweats, or unexplained weight loss.

To aid in the early diagnosis and management of TB.

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Who's behind your sample collection?

MEET YOUR PHLEBO

Certified & experienced professionals

Tata 1mg phlebotomists are DMLT / B.Sc MLT certified and have 1+ years of experience

Best in-class collections

Tata 1mg phlebotomists are trained for painless, single-prick hygienic sample collection

Comprehensive expertise

Other than sample collection, our phlebos are also skilled in first aid, ECG & BP monitoring

WHAT OUR CUSTOMERS ARE SAYING

Despite of very heavy rains, reached on time and did the work very professionally

~ Rahul Shrivastava

It didn't pain a 7 year old and hats off to the collector for being kind and extremely kid friendly

~ Khushnuma

Very nice person and soft spoken. I felt good talking to him even when I am in fever

~ Sourabh

New gloves new everything for each person, it's brilliant how meticulous and detailed your SOPs are.

~ Anita

My mother's veins are thin and slippery and hard to locate but Mohammed Usmani got it so fast, no pain

~ Rama Sampath

No pain at all while collecting blood sample. Fast, quick and properly trained staff.

~ Monalithatte

Sample collection agents was very professional and quick he was so organised with everything

~ Vaishali