
AFB DNA Detection by RT-PCR, Sputum near me in Howrah









The AFB DNA Detection by RT-PCR, Sputum test is a molecular diagnostic test that detects the genetic material (DNA) of Mycobacterium tuberculosis (TB) bacteria in a sputum sample using real-time PCR technology. It is widely available in Howrah with Tata 1mg labs at an affordable price.
The AFB DNA Detection by RT-PCR, Sputum test uses real-time PCR technology to find out the DNA of TB bacteria in a sputum sample. It is especially useful for individuals with persistent cough (>2 weeks), unexplained weight loss, fever, or other TB symptoms. It is also useful for those with close contacts of an individual diagnosed with TB, individuals with weak immune systems (e.g., HIV/AIDS, diabetes, cancer patients, or those on immunosuppressive drugs), and healthcare workers or individuals living or working in high-risk settings (hospitals, prisons, homeless shelters, etc.). It is noteworthy that the AFB DNA Detection by RT-PCR, Sputum test may not detect TB if the bacterial load is very low or if the sample is inadequate.
Collect sputum in a sterile, leak-proof container with a screw-cap lid. Before collecting the sample, rinse your mouth with clean water. Take two to three deep breaths, then cough deeply to bring up sputum from the lungs. Ensure you collect at least 2 ml of thick, yellowish (muco purulent) sputum. Avoid collecting only saliva or nasal secretions. As per National Tuberculosis Elimination Programme (NTEP) guidelines, testing requires two sputum samples. This may include a spot and early morning sample collected on consecutive days, or two spot samples collected one hour apart, for accurate diagnosis of tuberculosis (TB).








What does AFB DNA Detection by RT-PCR, Sputum measure?
The AFB DNA Detection by RT-PCR, Sputum test detects genetic material (DNA) of Mycobacterium tuberculosis, the bacteria that cause tuberculosis (TB), in a sputum sample.





FAQs related to AFB DNA Detection by RT-PCR, Sputum in Howrah
- Global tuberculosis report 2024 [Internet]. World Health Organization; [Accessed 14 Jul. 2025]. Available from:
- Press Information Bureau. World Tuberculosis (TB) Day – 2025: Towards a TB-Free India. Press Information Bureau; 2025 Mar 24. [Accessed 14 Jul. 2025]. Available from:
- National Health Mission. National Tuberculosis Elimination Programme (NTEP) Presentation for MD-NHM & PS. Ministry of Health & Family Welfare; 2021 Aug 26. [Accessed 14 Jul. 2025]. Available from:
- Dr.Oracle. What is the recommended treatment regimen for a patient requiring Anti-Tuberculosis Treatment (ATT)? Dr.Oracle; 2025. [Accessed 14 Jul. 2025]. Available from:
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