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AFB DNA Detection by RT-PCR, Sputum

AFB DNA Detection by RT-PCR, Sputum near me in Bhubaneswar

Also referred as
Tuberculosis DNA PCR - Qualitative
TB DNA Detection by Realtime PCR
181+ booked recently
For men & women
Earliest reports in
168 hours
Contains
1 test
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The AFB DNA Detection by RT-PCR, Sputum test is a molecular diagnostic test that detects the genetic material (DNA) of Mycobacterium tuberculosis (TB) bacteria in a sputum sample using real-time PCR technology.  It is widely available in Bhubaneswar with Tata 1mg labs at an affordable price.

The AFB DNA Detection by RT-PCR, Sputum test uses real-time PCR technology to find out the DNA of TB bacteria in a sputum sample. It is especially useful for individuals with persistent cough (>2 weeks), unexplained weight loss, fever, or other TB symptoms. It is also useful for those with close contacts of an individual diagnosed with TB, individuals with weak immune systems (e.g., HIV/AIDS, diabetes, cancer patients, or those on immunosuppressive drugs), and healthcare workers or individuals living or working in high-risk settings (hospitals, prisons, homeless shelters, etc.). It is noteworthy that the AFB DNA Detection by RT-PCR, Sputum test may not detect TB if the bacterial load is very low or if the sample is inadequate.

Collect sputum in a sterile, leak-proof container with a screw-cap lid. Before collecting the sample, rinse your mouth with clean water. Take two to three deep breaths, then cough deeply to bring up sputum from the lungs. Ensure you collect at least 2 ml of thick, yellowish (muco purulent) sputum. Avoid collecting only saliva or nasal secretions. As per National Tuberculosis Elimination Programme (NTEP) guidelines, testing requires two sputum samples. This may include a spot and early morning sample collected on consecutive days, or two spot samples collected one hour apart, for accurate diagnosis of tuberculosis (TB).

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Sputum
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Understanding AFB DNA Detection by RT-PCR, Sputum

Tuberculosis, or TB, is a contagious disease caused by bacteria called Mycobacterium tuberculosis. It primarily affects the lungs, but can also impact other parts of the body. It spreads through the air when an infected individual coughs, sneezes, or speaks, releasing droplets that can be inhaled by others. Early and accurate diagnosis is essential for timely treatment and limiting TB transmission. 

The AFB DNA Detection by RT-PCR, Sputum test uses real-time PCR technology to find out the DNA of TB bacteria in a sputum sample. It is especially useful for individuals with persistent cough (>2 weeks), unexplained weight loss, fever, or other TB symptoms. It is also useful for those with close contacts of an individual diagnosed with TB, individuals with weak immune systems (e.g., HIV/AIDS, diabetes, cancer patients, or those on immunosuppressive drugs), and healthcare workers or individuals living or working in high-risk settings (hospitals, prisons, homeless shelters, etc.).

It is noteworthy that the AFB DNA Detection by RT-PCR, Sputum test may not detect TB if the bacterial load is very low or if the sample is inadequate.

Collect sputum in a sterile, leak-proof container with a screw-cap lid. Before collecting the sample, rinse your mouth with clean water. Take two to three deep breaths, then cough deeply to bring up sputum from the lungs. Ensure you collect at least 2 ml of thick, yellowish (muco purulent) sputum. Avoid collecting only saliva or nasal secretions. As per National Tuberculosis Elimination Programme (NTEP) guidelines, testing requires two sputum samples. This may include a spot and early morning sample collected on consecutive days, or two spot samples collected one hour apart, for accurate diagnosis of tuberculosis (TB).

Laboratory test results may vary across laboratories. Do not self-medicate and always consult a doctor to understand the test results correctly. In some cases, repeat testing or additional confirmatory tests may be warranted.

Please note: This is an outsourced test. The sample for this test is collected by TATA 1MG and processed at a NABL accredited partnered lab.

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What does AFB DNA Detection by RT-PCR, Sputum measure?

The AFB DNA Detection by RT-PCR, Sputum test detects genetic material (DNA) of Mycobacterium tuberculosis, the bacteria that cause tuberculosis (TB), in a sputum sample.

FAQs related to AFB DNA Detection by RT-PCR, Sputum in Bhubaneswar

The AFB DNA Detection by RT-PCR, Sputum test helps detect genetic material (DNA) of Mycobacterium tuberculosis, the causative organism of tuberculosis (TB).
Individuals showing TB symptoms, those in contact with TB patients, or those with non-resolving respiratory infections.
Fasting is not required before this test. You can eat and drink normally as per your daily routine unless advised otherwise.
The AFB DNA Detection by RT-PCR, Sputum test is done by providing a sputum (mucus from your lungs) sample, which you will need to cough up into a sterile container. Once collected, the sample is sent to a laboratory where it undergoes testing to detect the presence of TB bacteria. If TB is confirmed, the same sample is then checked for resistance to rifampicin, a critical TB antibiotic.
Yes, the AFB DNA Detection by RT-PCR, Sputum test is better than a sputum smear, as PCR-based tests are more sensitive and provide drug resistance details.
Yes. In Bhubaneswar, you can schedule the AFB DNA Detection by RT-PCR, Sputum test near you with Tata 1mg Labs. The test is easy to book, cost-effective, and available with a comprehensive report.
Yes, home sample collection for the AFB DNA Detection by RT-PCR, Sputum test is readily available in Bhubaneswar. Tata 1mg Labs allows you to choose a date and time that suits your schedule for sample collection.
If you want to schedule the AFB DNA Detection by RT-PCR, Sputum test with Tata 1mg Labs in Bhubaneswar, the procedure is fairly easy and hassle-free. Follow these steps to book a test: 1). Visit the Lab Tests section on the Tata 1mg website. 2). Add the test you want to the cart. 3). Pick a time and date slot according to your convenience; however, do consider the necessary prerequisites while scheduling a test. 4). You will receive a notification with all the details once the test is scheduled.
Tata 1mg Labs is a pocket-friendly platform for booking lab tests in Bhubaneswar. The AFB DNA Detection by RT-PCR, Sputum test is available at an affordable price of Rs. 2060.
At Tata 1mg Labs, we understand the importance of timely results. We strive to provide fast turnaround times for test results, allowing you to get the information you need promptly. On average, we take 168 hours to provide the reports of the AFB DNA Detection by RT-PCR, Sputum test in Bhubaneswar.
Content created by
Written by:
Dr. Lipika Khurana
PGDHHM, BDS
Reviewed by:
Dr. Swati Gupta (Anand)
MD Lab Medicine, MBBS
Want to know moreRead our editorial policy
Last Updated6th Oct 2025

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AFB DNA Detection by RT-PCR, Sputum