Overview of Widal Test (Tube Agglutination)
What is Widal Test (Tube Agglutination)?
Why is Widal Test (Tube Agglutination) done?
To diagnose Enteric fever (Typhoid and Paratyphoid fever)
What does Widal Test (Tube Agglutination) Measure?
The Widal test measures the titres of antibody against the bacteria which cause Enteric fever.
Enteric fever is caused by bacterial infection and is of two types:
Typhoid fever - It is caused by Salmonella enterica serotype Typhi (S. Typhi).
Paratyphoid fever - It is caused by Salmonella enterica serotypes Paratyphi A (S. Paratyphi A), Paratyphi B (S. Paratyphi B) or Paratyphi C (S. Paratyphi C).
Typhoid and paratyphoid fever are generally acquired when you consume food or water, contaminated by feces of an acutely infected or convalescent person (recovering from disease) or a chronic, asymptomatic carrier. The incubation period (the time interval between exposure to an infection and the appearance of the first symptoms) of Enteric fever is 6-30 days.
Typhoid fever has a gradual onset. In the beginning, there is an increasing feeling of fatigue along with a fever which increases with every passing day. By the fourth or fifth day the fever may vary from low-grade to as high as 102°F–104°F (38°C–40°C). Known as the Stepladder fever pattern, this was once the hallmark symptom of Typhoid but is now witnessed in very few cases. The patient may also suffer from a headache, weakness, and loss of appetite. Abdominal pain, diarrhea, or constipation are common but not threatening. A transient rash of rose-colored spots can occasionally be seen on the trunk (Rose spots). The disease can last for a month, if untreated. Early diagnosis and treatment is important as life-threatening complications of typhoid fever generally occur after 2–3 weeks of illness and may include intestinal bleeding or perforation.
Paratyphoid fever is similar but often less severe than typhoid fever.
Widal test is an agglutination test that detects antibodies (agglutinins) in a blood sample against two antigens (O & H) of bacteria, Salmonella enterica. Agglutination refers to the visible clumping of particles when a particulate antigen combines with its antibody in the presence of optimum conditions for antigen-antibody reaction. When this test is performed in a test tube, it is called Tube agglutination and when it is carried out on a slide, it is called Slide agglutination. Widal test by Tube agglutination is recommended over Slide agglutination method. The antigens used in the test are “H” and “O” antigens of Salmonella Typhi and “H” antigen of S. Paratyphi.
Widal test should only be performed after the first week, as an antibody against “O” and “H” antigens of Salmonella start appearing in the serum at the end of the first week of fever. It is preferable to test two blood samples at an interval of 7 to 10 days to demonstrate rising antibody titres.
Preparation for Widal Test (Tube Agglutination)
- No special preparation required
Sample Type for Widal Test (Tube Agglutination)
Interpreting Widal Test (Tube Agglutination) results
If the antibody against Salmonella is present in patient’s serum, it will react with the respective antigen in the reagent and give visible agglutination (clumping) at the bottom of the test tube after 18 hrs.
The highest dilution of the patient’s serum sample that gives a visible agglutination with the Widal test antigen suspensions is the antibody titre.
The serum sample which shows the titre of 1:80 or more for O agglutination and 1:160 or more for H agglutination indicates active infection. A rise in titres (4-fold or more) in the two blood samples taken 10 days apart confirms the diagnosis. Please note that different labs may have different reference values for the interpretation of the Widal test.
The interpretation of a Widal test is greatly influenced by the nature and extent of the patient’s previous contact with typhoid antigens. It can be either due to clinical or subclinical infection with typhoid or related organisms or from typhoid vaccination. The lesser the extent of the previous contact, the greater is the usefulness of the Widal test.
Answers to Patient Concerns & Frequently Asked Questions (FAQs) about Widal Test (Tube Agglutination)
Frequently Asked Questions about Widal Test (Tube Agglutination)