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Hemoglobin (Hb) Electrophoresis by HPLC

800
4.7
NABL, ISO
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Overview
Interpreting Results

Overview of Hemoglobin (Hb) Electrophoresis by HPLC

What is Hemoglobin (Hb) Electrophoresis by HPLC?

This assay is useful in the diagnosis of disorders associated with abnormal forms of hemoglobin like sickle cell anemia, thalassemia.

Why is Hemoglobin (Hb) Electrophoresis by HPLC done?

  1. If you have signs or symptoms of hemolytic anemia like jaundice, fatigue, pale skin or splenomegaly 
  2. If your complete blood count and peripheral smear indicate that you may have an abnormal form of hemoglobin 
  3. It is commonly done as a part of newborn screening 
  4. It may used for prenatal screening if a parent is at high risk 

Preparation for Hemoglobin (Hb) Electrophoresis by HPLC

  • No special preparation required

Sample Type for Hemoglobin (Hb) Electrophoresis by HPLC

The sample type collected for Hemoglobin (Hb) Electrophoresis by HPLC is: Blood

Interpreting Hemoglobin (Hb) Electrophoresis by HPLC results

Interpretations

         All the results should be carefully interpreted in correlation with the patient's signs and symptoms and other laboratory findings. 

  • Presence of high level of sickle cell hemoglobin (HbS ) on HPLC denotes either sickle cell trait or sickle cell anemia 
  • Increased levels of fetal hemoglobin (HbF) on HPLC is associated with beta thalassemia major. Sometimes, it can also be seen in sickle cell anemia 
  • High HbA2 levels are mostly associated with beta thalassemia minor or trait. Recent studies say that HbA2 level between 4 to 9% is diagnostic of beta thalassemia trait 
  • Other less common hemoglobins which can be detected are hemoglobin D, J, G, M etc.

Provided By

NABL

ISO

Thyrocare Technologies Limited is India's first and most advanced Totally Automated Laboratory having its strong presence in more than 2000 cities / towns in India and internationally. Thyrocare is India's first fully automated diagnostic laboratory with a focus on providing quality at affordable costs to laboratories and hospitals in India and other countries. Thyrocare operates with a Centralized Processing Laboratory (CPL) in Mumbai - India for esoteric tests; and Regional Processing Laboratory in major metro cities of India and other parts of Asia. We have focus on strong technologies, strong brands and strong systems that enable all laboratories to give their clients the best of science and technology at an affordable cost. With a belief that 'Quality' is the heart of any intelligent management, Thyrocare became one of the first Indian diagnostic laboratories to obtain internationally renowned quality accreditations like ISO 9001-2000 rating as early as 2001, which is now escalated to ISO 9001:2008; NABL (National Accreditation Board for Testing and Calibration Laboratories) in 2005 and CAP (College of American Pathologists) certification in 2007.
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