Hemoglobin (Hb) Electrophoresis by HPLC

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Overview
Interpretations

Overview of Hemoglobin (Hb) Electrophoresis by HPLC

What is Hemoglobin (Hb) Electrophoresis by HPLC?

This assay is useful in the diagnosis of disorders associated with abnormal forms of hemoglobin like sickle cell anemia, thalassemia.

Sample Type

The sample type collected for Hemoglobin (Hb) Electrophoresis by HPLC is: Blood

Preparation for Hemoglobin (Hb) Electrophoresis by HPLC

  • No special preparation required

Why Get Tested for Hemoglobin (Hb) Electrophoresis by HPLC?

  1. If you have signs or symptoms of hemolytic anemia like jaundice, fatigue, pale skin or splenomegaly 
  2. If your complete blood count and peripheral smear indicate that you may have an abnormal form of hemoglobin 
  3. It is commonly done as a part of newborn screening 
  4. It may used for prenatal screening if a parent is at high risk 

What Results of Hemoglobin (Hb) Electrophoresis by HPLC mean?

Interpretations

         All the results should be carefully interpreted in correlation with the patient's signs and symptoms and other laboratory findings. 

  • Presence of high level of sickle cell hemoglobin (HbS ) on HPLC denotes either sickle cell trait or sickle cell anemia 
  • Increased levels of fetal hemoglobin (HbF) on HPLC is associated with beta thalassemia major. Sometimes, it can also be seen in sickle cell anemia 
  • High HbA2 levels are mostly associated with beta thalassemia minor or trait. Recent studies say that HbA2 level between 4 to 9% is diagnostic of beta thalassemia trait 
  • Other less common hemoglobins which can be detected are hemoglobin D, J, G, M etc.

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