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Hemoglobin (Hb) Electrophoresis by HPLC

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Overview
Test Results & Interpretations

Overview of Hemoglobin (Hb) Electrophoresis by HPLC

Test Description

This assay is useful in the diagnosis of disorders associated with abnormal forms of hemoglobin like sickle cell anemia, thalassemia.

Why Get Tested

  1. If you have signs or symptoms of hemolytic anemia like jaundice, fatigue, pale skin or splenomegaly 
  2. If your complete blood count and peripheral smear indicate that you may have an abnormal form of hemoglobin 
  3. It is commonly done as a part of newborn screening 
  4. It may used for prenatal screening if a parent is at high risk 

Precautions

  • No special preparation required

What Results of Hemoglobin (Hb) Electrophoresis by HPLC mean?

Interpretations

         All the results should be carefully interpreted in correlation with the patient's signs and symptoms and other laboratory findings. 

  • Presence of high level of sickle cell hemoglobin (HbS ) on HPLC denotes either sickle cell trait or sickle cell anemia 
  • Increased levels of fetal hemoglobin (HbF) on HPLC is associated with beta thalassemia major. Sometimes, it can also be seen in sickle cell anemia 
  • High HbA2 levels are mostly associated with beta thalassemia minor or trait. Recent studies say that HbA2 level between 4 to 9% is diagnostic of beta thalassemia trait 
  • Other less common hemoglobins which can be detected are hemoglobin D, J, G, M etc.

Provided By

NABL

Welcome to Asian Diagnostics, an organization that has been successfully delivering high quality medical Diagnostic Services in Karnataka and South India for over 15 years. Established in 1999, it is based out of Bangalore with branches in Mysore, Hassan and Vijayapura. Asian Diagnostics has continued to evolve over the years to cater to the needs of doctors and patients alike and offers an extensive range of clinical diagnostic tests making it a one-stop destination for the best in Diagnostic Services. Asian Diagnostics is proud to have successfully completed the NABL accreditation and is one of the few NABL Accredited Labs in Bangalore. Operated by a team of highly skilled and committed professionals, it has remained committed to its objective of providing high quality, accurate and reliable diagnostic services at affordable prices to its ever growing customer base. Asian diagnostics is currently THE LARGEST ALLERGY TEST CENTER IN INDIA. It is the first laboratory in the state to perform quantitative 'Food Intolerance' tests

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